Blood serum is a complex body fluid that contains various proteins ranging in concentration
over at least 9 orders of magnitude. Using a combination of mass spectrometry technologies …

Proteomics became a key tool for the study of biological systems. The comparison between
two different physiological states allows unravelling the cellular and molecular mechanisms …

One of the most challenging contemporary research endeavors is the mapping of proteins
and establishing their linkages to normal and pathological conditions. The availability of …

One of the most effective methods for the direct identification of proteins from complex
mixtures without first having to resolve them by polyacrylamide gel electrophoresis is to …

The proteome is a dynamic system in which each protein has interconnected properties—
dimensions—that together contribute to the phenotype of a cell. Measuring these properties …

The proteomic analysis of plasma and serum samples represents a formidable challenge
due to the presence of a few highly abundant proteins such as albumin and …

In recent years, several proteomic methodologies have been developed that now make it
possible to identify, characterize, and comparatively quantify the relative level of expression …

Large-scale proteomics often employs two orthogonal separation methods to fractionate
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …

Technological developments in proteomics have had a dramatic impact on biology in recent
years. One of these developments—named multidimensional protein identification …

In-depth, reproducible coverage of complex proteomes is challenging because the
complexity of tryptic digests subjected to LC-MS/MS analysis frequently exceeds mass …