Protein copy number is distributed from 7 to 8 orders of magnitude in cells and probably up
to 12 orders of magnitude in plasma. Classical silver‐stained two‐dimensional …

Proteomic analysis can be hampered by the large concentration distribution of proteins.
Immunoaffinity techniques have been applied to selectively remove high abundant proteins …

There are probably no biological samples that did more to spur interest in proteomics than
serum and plasma. The belief was that comparing the proteomes of these samples obtained …

Discovery-based proteomics has found its place in nearly every facet of biological research.
A key objective of this approach is to maximize sequence coverage for proteins across a …

Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent
proteomic approach due to its high-throughput separations and accurate mass detection …

Proteomics has its origins in two-dimensional gel electrophoresis (2-DE), a technique
developed more than twenty years ago. 2-DE has a high-resolution capacity, and was …

Proteins are essential constituents of all organisms, and they participate in virtually every
process within cells. These macromolecules are found in roles that are enzymatic …

Mass spectrometry-based proteomics has become the tool of choice for identifying and
quantifying the proteome of an organism. Though recent years have seen a tremendous …

Proteome analysis represents significant challenges to the existing sample preparation
techniques. Traditional methods, such as two‐dimensional electrophoresis, typically …

166 TC Hunter et al./J. Chromatogr. B 782 (2002) 165–181 the human genome sequence
[2], and the completion ing the recent advent of commercial hybrid instruof the genomic …