In the past decade, major developments in instrumentation and methodology have been
achieved in proteomics. For proteome investigations of complex biological samples derived …

After the first introduction of the concept of “proteome” more than 10 years ago, large-scale
studies of protein expression, localization, activities and interactions have gained an …

Two‐dimensional remains one of the main experimental approaches in proteome analysis.
However, comigration of protein leads to several limitations: lack of accuracy in protein …

Publisher Summary Proteomics is the analysis of the entire protein complement of a cell,
tissue, or organism under a specific, defined set of conditions. In its present state, it is …

Sample preparation is a fundamental step in the proteomics workflow. However, it is not
easy to find compiled information updating this subject. In this paper, the strategies and …

The systematic analysis of biological processes requires an understanding of the
quantitative expression patterns of proteins, their interacting partners and their subcellular …

Highly complex protein mixtures can be directly analyzed after proteolysis by liquid
chromatography coupled with tandem mass spectrometry (LC− MS/MS). In this paper, we …

Proteomics can be thought of as an attempt to understand the information encoded in
genomic sequences from the perspective of proteins; ie the structure, function and regulation …

A two-dimensional liquid chromatographic system is described here which uses size-
exclusion liquid chromatography (SEC) followed by reversed-phase liquid chromatography …

Two‐dimensional liquid chromatography separation (2‐DL), based on chromatofocusing for
first dimension and hydrophobicity for second, can be used as a complementary method to …