Bovine oocytes are arrested at the prophase of first meiotic cell cycle. Meiosis resumes in
oocytes of pre‐ovulatory follicles upon LH surge. However, oocytes from secondary follicles …

An in vitro culture system in which two types of cell from ovarian follicular walls were
attached to opposite surfaces of a collagen membrane was developed. The effect of …

Invasive adenylate cyclase (iAC) reversibly inhibits spontaneous maturation of cumulus-
enclosed bovine oocytes by increasing the intracellular concentration of cAMP,[cAMP] i. In …

The effect of adenylate cyclase stimulation via the components of the enzyme on nuclear
maturation in bovine cumulus-enclosed and zona-free oocytes was examined. The …

Meiotic maturation occurs spontaneously when bovine oocytes are removed from their
follicles and cultured in vitro. This natural phenomenon in mammals has greatly facilitated …

The rate of spontaneous meiotic maturation and the period of commitment to this process
were determined in bovine oocytes devoid of surounding cumulus cells, cultured in …

To prolong the culture of oocytes, it is essential to know how the follicle maintains meiotic
arrest. This study was undertaken to evaluate the short-term effects (24 h) of the co-culture of …

We evaluated the importance of the attachment of the oocyte to the granulosa layer, using co-
culture of follicular hemi-sections with groups of 10 COCs (cumulus-oocyte complexes) or 10 …

The effects of high intracellular cAMP concentrations ([cAMP] i) on germinal vesicle
maintenance of bovine cumulus–oocyte complexes were investigated, using 8-bromo-3 …

The oocytes of cattle are not as sensitive as those of laboratory animals to purines, cAMP, or
follicular extracts. To study the resumption of meiosis, a method is needed that is capable of …