A detailed transcriptional analysis of the conjugative transposon Tn916 was carried out,
which revealed that transcription of the transfer functions requires excision of the element …

Nucleoid-associated proteins (NAPs) such as IHF, HU, Fis, and H-NS alter the topology of
bound DNA and may thereby affect accessibility of DNA to repair and recombination …

In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam-cells
than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and …

Global transposon mutagenesis is a valuable tool for identifying genes required for cell
viability. Here we present a global analysis of the orientation of viable Tn 5-Puror (Tn 5 …

Bacteria use two‐component signal transduction pathways to sense both extracellular and
intracellular environment and to coordinate cellular events according to changing …

Transposable elements are important in genome dynamics and evolution. Bacterial insertion
sequences (IS) constitute a major group in number and impact. Understanding their role in …

The movement of the bacterial insertion sequence IS1 often generates cointegrate structures
in which donor and target replicons are connected by direct repeats of IS1. The experiments …

The binding of Tn916 Xis protein to its specific sites at the left and right ends of the
transposon was compared using gel mobility shift assays. Xis formed two complexes with …

ABSTRACT IS 911 is a bacterial insertion sequence composed of two consecutive
overlapping open reading frames (ORFs [orfA and orfB]) encoding the transposase (OrfAB) …