IS911‐mediated intramolecular transposition is naturally temperature sensitive

L Haren, M Bétermier, P Polard… - Molecular …, 1997 - Wiley Online Library
It is shown here that the bacterial insertion sequence IS911 exhibits a temperature‐sensitive
transposition phenotype. Previous results have demonstrated that elevated levels of the …

Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi

AA Hidalgo, AN Trombert, JC Castro-Alonso… - Genetics, 2004 - academic.oup.com
We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon
Tn 10 dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP …

IS903 transposase mutants that suppress defective inverted repeats

NP Tavakoli, KM Derbyshire - Molecular microbiology, 1999 - Wiley Online Library
The inverted repeats (IRs) of the insertion element IS903 are composed of two functional
regions. An inner region, consisting of basepairs 6–18, is the transposase binding site. The …

Genetic and transcriptional analysis of a regulatory region in streptococcal conjugative transposon Tn5252

P Srinivas, MN Vijayakumar - Plasmid, 2000 - Elsevier
In an attempt to increase our understanding of the mechanisms of conjugal transposition
among gram-positive bacteria, we analyzed the genetic and structural properties of a 1.2-kb …

[HTML][HTML] Tipping the balance between replicative and simple transposition

NP Tavakoli, KM Derbyshire - The EMBO Journal, 2001 - embopress.org
The bacterial insertion sequence IS903 has the unusual ability to transpose both
replicatively and non‐replicatively. The majority of products are simple insertions, while co …

Facilities for transcription and mobilization of an exon-less bacterial group II intron nested in transposon TnMERI1

MF Chien, CC Huang, T Kusano, G Endo - Gene, 2008 - Elsevier
Transcription and mobilization facilities of a group II intron, B. me. I1, which was classified
within a group IIB subclass and nested in a broad-spectrum mercury resistance transposon …

Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an …

H Yigit, WS Reznikoff - Journal of bacteriology, 1997 - Am Soc Microbiol
Tn5 transposase (Tnp) overproduction is lethal to Escherichia coli. Tnp overproduction
causes cell filamentation, abnormal chromosome segregation, and an increase in …

Tn4371: A Modular Structure Encoding a Phage-like Integrase, aPseudomonas-like Catabolic Pathway, and RP4/Ti-like Transfer Functions

C Merlin, D Springael, A Toussaint - Plasmid, 1999 - Elsevier
Tn4371is a 55-kb catabolic transposon originally isolated fromRalstonia eutrophaA5 that
encodes enzymes catalyzing the complete degradation of biphenyl. Unlike previously …

First biochemical steps on bacterial transposition pathways

C Guynet, E Nicolas, B Ton-Hoang, JY Bouet… - … Gene Transfer: Methods …, 2020 - Springer
Transposons are found in a wide variety of forms throughout the prokaryotic world where
they actively contribute to the adaptive strategies of bacterial communities and hence, to the …

Co-regulation by bent DNA. Functional substitutions of the integration host factor site at sigma 54-dependent promoter Pu of the upper-TOL operon by intrinsically …

J Perez-Martin, KN Timmis, V De Lorenzo - Journal of Biological Chemistry, 1994 - Elsevier
The role of integration host factor (IHF) in the regulation of the sigma 54-dependent promoter
Pu of the TOL plasmid of Pseudomonas putida has been examined. We have selected in …