Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering
H Altae-Tran, S Kannan, AJ Suberski, KS Mears… - Science, 2023 - science.org
Microbial systems underpin many biotechnologies, including CRISPR, but the exponential
growth of sequence databases makes it difficult to find previously unidentified systems. In …
growth of sequence databases makes it difficult to find previously unidentified systems. In …
Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
We present multiplex Digenome-seq to profile genome-wide specificities of up to 11
CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human …
CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost. Cell-free human …
CRISPR-Cas: new tools for genetic manipulations from bacterial immunity systems
W Jiang, LA Marraffini - Annual review of microbiology, 2015 - annualreviews.org
Prokaryotic CRISPR-Cas loci encode proteins that function as an adaptive immune system
against infectious viruses and plasmids. Immunity is mediated by Cas nucleases and small …
against infectious viruses and plasmids. Immunity is mediated by Cas nucleases and small …
A robust CRISPR interference gene repression system in Pseudomonas
Pseudomonas spp. are widely used model organisms in different areas of research. Despite
the relevance of Pseudomonas in many applications, the use of protein depletion tools in …
the relevance of Pseudomonas in many applications, the use of protein depletion tools in …
AcrFinder: genome mining anti-CRISPR operons in prokaryotes and their viruses
Anti-CRISPR (Acr) proteins encoded by (pro) phages/(pro) viruses have a great potential to
enable a more controllable genome editing. However, genome mining new Acr proteins is …
enable a more controllable genome editing. However, genome mining new Acr proteins is …
Characterizing leader sequences of CRISPR loci
OS Alkhnbashi, SA Shah, RA Garrett… - …, 2016 - academic.oup.com
Abstract Motivation The CRISPR-Cas system is an adaptive immune system in many
archaea and bacteria, which provides resistance against invading genetic elements. The …
archaea and bacteria, which provides resistance against invading genetic elements. The …
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
T Karvelis, G Gasiunas, J Young, G Bigelyte… - Genome biology, 2015 - Springer
To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro
method for the simultaneous examination of guide RNA and protospacer adjacent motif …
method for the simultaneous examination of guide RNA and protospacer adjacent motif …
Sequence determinants of improved CRISPR sgRNA design
The CRISPR/Cas9 system has revolutionized mammalian somatic cell genetics. Genome-
wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated …
wide functional screens using CRISPR/Cas9-mediated knockout or dCas9 fusion-mediated …
Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting
Background Genome editing by CRISPR-Cas9 technology allows large-scale screening of
gene essentiality in cancer. A confounding factor when interpreting CRISPR-Cas9 screens …
gene essentiality in cancer. A confounding factor when interpreting CRISPR-Cas9 screens …
Mobile CRISPR/Cas-Mediated Bacteriophage Resistance in Lactococcus lactis
Lactococcus lactis is a biotechnological workhorse for food fermentations and potentially
therapeutic products and is therefore widely consumed by humans. It is predominantly used …
therapeutic products and is therefore widely consumed by humans. It is predominantly used …