Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA bioinformatics, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Single-cell RNA-seq technologies and related computational data analysis

G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

Single-cell RNA sequencing and its combination with protein and DNA analyses

JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

Critical downstream analysis steps for single-cell RNA sequencing data

Z Zhang, F Cui, C Lin, L Zhao, C Wang… - Briefings in …, 2021 - academic.oup.com
Single-cell RNA sequencing (scRNA-seq) has enabled us to study biological questions at
the single-cell level. Currently, many analysis tools are available to better utilize these …

Experimental considerations for single-cell RNA sequencing approaches

QH Nguyen, N Pervolarakis, K Nee… - Frontiers in cell and …, 2018 - frontiersin.org
Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular
heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell …

Full-length single-cell RNA sequencing with smart-seq2

S Picelli - Single Cell Methods: Sequencing and Proteomics, 2019 - Springer
In the last few years single-cell RNA sequencing (scRNA-seq) has enabled the investigation
of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as …

Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data

TS Andrews, VY Kiselev, D McCarthy, M Hemberg - Nature protocols, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database

L Zappia, B Phipson, A Oshlack - PLoS computational biology, 2018 - journals.plos.org
As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the
number of tools designed to analyse these data has dramatically increased. Navigating the …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …