Various times of in vitro maturation of oocytes, and three methods of separating
spermatozoa from frozen-thawed semen (Percoll density-gradient centrifugation, swim-up …

The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated
boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered …

Experiments were carried out to study morphological changes temporally associated with in
vitro fertilization (IVF) of prepubertal goat oocytes and to elucidate some of the abnormalities …

Ejaculates from 3 young boars were collected on 4 occasions as a series of separate 15-ml
fractions. The contribution of different fractions of these ejaculates to observed variability in …

The objectives of these experiments were to characterize separation of frozen-thawed
bovine spermatozoa on a Percoll gradient and then to compare sperm separation by either a …

This study was designed to investigate the effect of sperm exposure time on the fertilization
rate and subsequent developmental capacity of bovine oocytes matured in vitro. Cumulus …

Bovine oocytes matured in vivo or in vitro were evaluated after sperm-oocyte incubation for
frequency of sperm penetration, frequency of male pronuclei formation, and embryonic …

Follicular cumulus-enclosed goat oocytes were matured in vitro in the presence of granulosa
cells, follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol-17β. While …

Two techniques for the preparation of boar spermatozoa for in vitro fertilization were studied:
a simple washing procedure and centrifugation on a discontinuous Percoll gradient. Their …

Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …