We have used in vitro mutagenesis and gene replacement to study the function of the
nucleotide-binding domain (NBD) of γ-tubulin in Tetrahymena thermophila. In this study, we …

Basal bodies and centrioles are conserved microtubule-based organelles the improper
assembly of which leads to a number of diseases, including ciliopathies and cancer. Tubulin …

Background The multi-tubulin hypothesis proposes that each tubulin isotype performs a
unique role, or subset of roles, in the universe of microtubule function (s). To test this …

In ciliates, different microtubular structures are nucleated from diverse Microtubule
Organizing Centers (MTOCs). γ-Tubulin is a tubulin superfamily member that plays an …

Microtubule assembly is initiated in vivo by γ-tubulin complexes. Cytoplasmic γ-tubulin
complexes are targeted to centrosomes or to other microtubule organizing centers (MTOCs) …

We have isolated suppressors of the deletion allele of δ-tubulin, uni3-1, in the biflagellate
green alga Chlamydomonas reinhardtii. The deletion of δ-tubulin produces cells that …

The γ-tubulin ring complex (γTuRC), purified from the cytoplasm of vertebrate and
invertebrate cells, is a microtubule nucleator in vitro. Structural studies have shown that …

We have cloned and sequenced the two β-tubulin genes of the ciliated protozoan
Tetrahymena thermophila. The two genes encode identical 443 amino acid peptides which …

Abstract γ-Tubulin is a conserved component of all microtubule-organizing centres and is
required for these organelles to nucleate microtubule polymerization. However, the …

Polyglycylation occurs through the post-translational addition of a polyglycine peptide to the
γ-carboxyl group of glutamic acids near the C terminus of α-and β-tubulin, and has been …