In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for
the characterization of proteomic samples. This workflow combines protein fractionation …

Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …

Proteomics, the characterization of the proteome, is conceptually simple but technically
challenging. Development of such technologies as mass spectrometry, multidimensional …

The routine detection of low abundance components in complex samples for detailed
proteomics analysis continues to be a challenge. Whilst the potential of multidimensional …

Multidimensional chromatography coupled to tandem mass spectrometry is an emerging
technique for the analysis of proteomes and is rapidly being implemented by many …

As a complementary approach to two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE), multi-dimensional chromatography separation methods have been widely applied in …

The limitations of 2-D gels for global proteomics have encouraged the development of
alternative approaches for identifying proteins in complicated mixtures, and determining …

Mass spectrometry used in combination with a wide variety of separation methods is the
principal methodology for proteomics. In bottom-up approach, proteins are cleaved with a …

Top–down proteomics (TDP) identifies, quantifies, and characterises proteins at the intact
proteoform level in complex biological samples to understand proteoform function and …

We investigated the combination of weak anion exchange (WAX) fractionation and on-line
reversed-phase liquid chromatography (RPLC) separation using a 12 T FTICR mass …