In the crystal structure of a substrate complex, the side chains of residues Asn 279, Tyr 271,
and Arg 283 of DNA polymerase β are within hydrogen bonding distance to the bases of the …

Conserved site-directed mutations were introduced into the second most conserved amino
acid region, region II, of the human DNA polymerase alpha catalytic subunit. These mutants …

Publisher Summary The chapter discusses the purification and domain mapping of
mammalian DNA polymerase β (Pol β) and focuses on the isolation of recombinant Pol β …

DNA polymerase β (pol β) is the smallest and least complex DNA polymerase. The structure
of the enzyme is well understood, but little is known about its catalytic properties, particularly …

Revised Manuscript Received March 1, 1991 abstract: The sequence Gly-Asp-Met-Asp,
spanning positions 189-192 of rat DNA polymerase 0, is similar to the sequence motif Gly …

DNA polymerase (pol) λ is homologous to pol β and has intrinsic polymerase and terminal
transferase activities. However, nothing is known about the amino acid residues involved in …

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health,
Bethesda, Maryland 20892 Received May 17, 1990; Revised Manuscript Received June 5 …

Department of Biochemistry, Kanazawa Medical University, Uchinada, Kahoku-gun,
Ishikawa 920-02, Japan, Laboratory of Cell Biology, Aichi Cancer Center Research Institute …

Interactions between the isolated 8-kDa domain of the rat DNA polymerase β and DNA have
been studied, using the quantitative fluorescence titration technique. The obtained results …

The kinetics of nucleotide incorporation into 24/36-mer primer/template DNA by purified fetal
calf thymus DNA polymerase (pol) δ was examined using steady-state and pre-steady-state …