OBJECTIVE: To develop a rapid, sensitive, and reproducible hypoosmotic swelling test kit
for the assessment of plasma membrane integrity of human sperm in vitro. DESIGN …

The hypo‐osmotic swelling (HOS) test has been proposed as a useful assay for evaluation
of the functional competence of the human sperm membranes. To assess this further, the …

Routine methods for the evaluation of sperm quality are not sufficiently useful to determine
the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to …

Objective:[1] To examine the relationship between sperm membrane integrity and motion
parameters before and after cryopreservation;[2] to determine the capacity of the membrane …

In summary, the hypoosmotic viability parameter was significantly correlated with the
outcome of the thawed sperm motility. The prefreeze supravital staining for sperm viability …

Objective: To report a high fertilization rate after intracytoplasmic sperm injection (ICSI) in
patients with 100% nonmotile spermatozoa selected by a simple modified hypo-osmotic …

Viable, healthy sperm are preferred for oocyte fertilization with intracytoplasmic sperm
injection. Currently, motility is the most widely applied measure of sperm viability. However …

Aim To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify
viable, non-motile sperm. Methods Semen samples from 20 men with severe …

A modified hypo-osmotic solution was used to select viable ejaculated and testicular
spermatozoa to perform intracytoplasmic sperm injection (ICSI) in 27 treatment cycles from …

The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the
membrane integrity of spermatozoa of various domestic animals including cattle, horses …