We examined transgenic‐cattle production by DNA microinjection into 1‐, 2‐, and 4‐cell
embryos, analyzing the impact on calf size and subsequent viability. Embryos were either …

This study was designed to investigate the potential use of in vitro matured, in vitro fertilized
bovine zygotes for producing transgenic calves by microinjection of foreign DNA. In …

We have combined gene transfer, by microinjection, with 'in vitro'embryo production
technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected …

The objectives of these experiments were: 1) to determine an effective culture method for
production of transferable bovine embryos following exogenous DNA microinjection; 2) to …

Results of the present analysis confirm that sex selection may be inadvertently performed
with in vitro fertilized-in vitro cultured (IVF-IVC) bovine embryos. We have shown that under …

Bovine zygotes produced by in vitro maturation–in vitro fertilization (IVM–IVF) were
examined for their potential to serve as recipients of transgenes. Pronuclei, which were …

Sheep embryos microinjected with DNA (RSVCAT: fusion gene of Rous Sarcoma Virus
promoter and the bacterial chloramphenicol acetyl transferase structural gene) were co …

Microinjection and in vitro culture procedures were developed to produce transgenic bovine
embryos after in vitro fertilization of in vitro matured oocytes. In Experiment I, zygotes were …

The production of transgenic cattle presents a number of unique challenges not
encountered in other species. First, the survival of microinjected zygotes is low; only 15% in …

This study was designed to measure the survival and development of pronuclear stage
bovine zygotes after microinjection with DNA and either culture in vitro or incubation in the …