Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength

I Testa, CA Wurm, R Medda, E Rothermel… - Biophysical journal, 2010 - cell.com
Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a
mechanism of fluorescence inhibition. This mechanism is implemented such that features …

Photoswitching microscopy with standard fluorophores

S van de Linde, R Kasper, M Heilemann, M Sauer - Applied Physics B, 2008 - Springer
We introduce far-field subdiffraction-resolution fluorescence imaging based on
photoswitching of individual standard fluorophores in air-saturated solution. Here …

Photoswitches: Key molecules for subdiffraction‐resolution fluorescence imaging and molecular quantification

M Heilemann, P Dedecker, J Hofkens… - Laser & Photonics …, 2009 - Wiley Online Library
Optical microscopes, often referred to as 'light microscopes', use visible light and a system of
lenses to provide us with magnified images of small samples. Combined with highly …

Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

M Bossi, J Folling, VN Belov, VP Boyarskiy… - Nano …, 2008 - ACS Publications
By combining the photoswitching and localization of individual fluorophores with
spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging …

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

T Grotjohann, I Testa, M Leutenegger, H Bock… - Nature, 2011 - nature.com
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for
decades, but the recent breaking of the diffraction resolution barrier by sequentially …

Optical nanoscopy: from acquisition to analysis

TJ Gould, ST Hess, J Bewersdorf - Annual review of biomedical …, 2012 - annualreviews.org
Recent advances in far-field microscopy have demonstrated that fluorescence imaging is
possible at resolutions well below the long-standing diffraction limit. By exploiting …

Lens-based fluorescence nanoscopy

C Eggeling, KI Willig, SJ Sahl, SW Hell - Quarterly reviews of …, 2015 - cambridge.org
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

Coordinate-targeted fluorescence nanoscopy with multiple off states

JG Danzl, SC Sidenstein, C Gregor, NT Urban… - Nature …, 2016 - nature.com
Far-field super-resolution fluorescence microscopy discerns fluorophores residing closer
than the diffraction barrier by briefly transferring them in different (typically ON and OFF) …

Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes

M Bates, B Huang, X Zhuang - Current opinion in chemical biology, 2008 - Elsevier
A new form of super-resolution fluorescence microscopy has emerged in recent years,
based on the high accuracy localization of individual photo-switchable fluorescent labels …

Multicolor super-resolution imaging with photo-switchable fluorescent probes

M Bates, B Huang, GT Dempsey, X Zhuang - Science, 2007 - science.org
Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a
spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however …