Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength
I Testa, CA Wurm, R Medda, E Rothermel… - Biophysical journal, 2010 - cell.com
Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a
mechanism of fluorescence inhibition. This mechanism is implemented such that features …
mechanism of fluorescence inhibition. This mechanism is implemented such that features …
Photoswitching microscopy with standard fluorophores
We introduce far-field subdiffraction-resolution fluorescence imaging based on
photoswitching of individual standard fluorophores in air-saturated solution. Here …
photoswitching of individual standard fluorophores in air-saturated solution. Here …
Photoswitches: Key molecules for subdiffraction‐resolution fluorescence imaging and molecular quantification
M Heilemann, P Dedecker, J Hofkens… - Laser & Photonics …, 2009 - Wiley Online Library
Optical microscopes, often referred to as 'light microscopes', use visible light and a system of
lenses to provide us with magnified images of small samples. Combined with highly …
lenses to provide us with magnified images of small samples. Combined with highly …
Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species
By combining the photoswitching and localization of individual fluorophores with
spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging …
spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging …
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
T Grotjohann, I Testa, M Leutenegger, H Bock… - Nature, 2011 - nature.com
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for
decades, but the recent breaking of the diffraction resolution barrier by sequentially …
decades, but the recent breaking of the diffraction resolution barrier by sequentially …
Optical nanoscopy: from acquisition to analysis
TJ Gould, ST Hess, J Bewersdorf - Annual review of biomedical …, 2012 - annualreviews.org
Recent advances in far-field microscopy have demonstrated that fluorescence imaging is
possible at resolutions well below the long-standing diffraction limit. By exploiting …
possible at resolutions well below the long-standing diffraction limit. By exploiting …
Lens-based fluorescence nanoscopy
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …
Coordinate-targeted fluorescence nanoscopy with multiple off states
JG Danzl, SC Sidenstein, C Gregor, NT Urban… - Nature …, 2016 - nature.com
Far-field super-resolution fluorescence microscopy discerns fluorophores residing closer
than the diffraction barrier by briefly transferring them in different (typically ON and OFF) …
than the diffraction barrier by briefly transferring them in different (typically ON and OFF) …
Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes
A new form of super-resolution fluorescence microscopy has emerged in recent years,
based on the high accuracy localization of individual photo-switchable fluorescent labels …
based on the high accuracy localization of individual photo-switchable fluorescent labels …
Multicolor super-resolution imaging with photo-switchable fluorescent probes
Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a
spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however …
spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however …