Time‐domain whole‐field fluorescence lifetime imaging with optical sectioning

MJ Cole, J Siegel, SED Webb, R Jones… - Journal of …, 2001 - Wiley Online Library
A whole‐field time‐domain fluorescence lifetime imaging (FLIM) microscope with the
capability to perform optical sectioning is described. The excitation source is a mode‐locked …

A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning

SED Webb, Y Gu, S Lévêque-Fort, J Siegel… - Review of Scientific …, 2002 - pubs.aip.org
This article describes a wide-field time-domain fluorescence lifetime imaging FLIM
microscope with optical sectioning. The FLIM system utilizes a wide-field time-gated optical …

Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

Fluorescence lifetime imaging microscopy (FLIM): spatial resolution of microstructures on the nanosecond time scale

TWJ Gadella Jr, TM Jovin, RM Clegg - Biophysical chemistry, 1993 - Elsevier
A frequency domain fluorescence lifetime imaging microscope (FLIM) has been developed.
A continuous wave laser excitation source of an epi-illumination fluorescence microscope is …

φFLIM: a new method to avoid aliasing in frequency‐domain fluorescence lifetime imaging microscopy

EB Van Munster, TWJ Gadella Jr - Journal of microscopy, 2004 - Wiley Online Library
In conventional wide‐field frequency‐domain fluorescence lifetime imaging microscopy
(FLIM), excitation light is intensity‐modulated at megahertz frequencies. Emitted …

Whole-field optically sectioned fluorescence lifetime imaging

MJ Cole, J Siegel, SED Webb, R Jones, K Dowling… - Optics Letters, 2000 - opg.optica.org
Whole-field optically sectioned fluorescence lifetime imaging clickable element to expand a
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Innovating lifetime microscopy: a compact and simple tool for life sciences, screening, and diagnostics

A Esposito, HC Gerritsen, T Oggier… - Journal of …, 2006 - spiedigitallibrary.org
Fluorescence lifetime imaging microscopy (FLIM) allows the investigation of the
physicochemical environment of fluorochromes and protein-protein interaction mapping by …

Light-field tomographic fluorescence lifetime imaging microscopy

Y Ma, J Park, L Huang, C Sen, S Burri… - Proceedings of the …, 2024 - pnas.org
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that
enables the visualization of biological samples at the molecular level by measuring the …

All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

A Esposito, T Oggier, HC Gerritsen, F Lustenberger… - Optics express, 2005 - opg.optica.org
Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is
increasingly being used in the life sciences during the past decades. However, a broader …

Fluorescence lifetime system for microscopy and multiwell plate imaging with a blue picosecond diode laser

DS Elson, J Siegel, SED Webb, S Lévêque-Fort… - Optics letters, 2002 - opg.optica.org
We report a wide-field fluorescence lifetime imaging (FLIM) system that uses a blue
picosecond pulsed diode laser as the excitation source. This represents a significant …