Both carboxy-terminal tails of α-and β-tubulin are essential, but either one will suffice

J Duan, MA Gorovsky - Current biology, 2002 - cell.com
Microtubules (MTs) are organized into distinct systems essential for cell shape, movement,
intracellular transport, and division [1]. Electron crystallographic analyses [2] provide little …

γ-Tubulin in trypanosomes: molecular characterisation and localisation to multiple and diverse microtubule organising centres

V Scott, T Sherwin, K Gull - Journal of cell science, 1997 - journals.biologists.com
ABSTRACT A genomic clone from Trypanosoma brucei, which contains a full length γ-
tubulin gene, was isolated using degenerate oligonucleotide primers. The sequence of this …

Mammalian cilia function is independent of the polymeric state of tubulin glycylation

SJY Dossou, MH Bré… - Cell motility and the …, 2007 - Wiley Online Library
Polyglycylation is a polymeric post‐translational modification of tubulin that is ubiquitous and
widely present in cilia and flagella. It consists of the addition of highly variable numbers of …

Sequence analysis and immunofluorescence study of α‐ and β‐tubulins in Reticulomyxa filosa: Implications of the high degree of β2‐tubulin divergence

S Linder, M Schliwa… - Cell motility and the …, 1997 - Wiley Online Library
We have cloned and sequenced 2 α‐and 2 β‐tubulin isoforms from the giant freshwater
amoeba Reticulomyxa filosa. The microtubules of this organism exhibit some unusual …

Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins

S Kubo, CS Black, E Joachimiak, SK Yang… - Nature …, 2023 - nature.com
Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory
functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central …

Ciliate Microtubule Diversities: Insights from the EFBTU3 Tubulin in the Antarctic Ciliate Euplotes focardii

S Pucciarelli, D Sparvoli, P Ballarini, A Piersanti… - Microorganisms, 2022 - mdpi.com
Protozoans of the Phylum Ciliophora (ciliates) assemble many diverse microtubular
structures in a single cell throughout the life cycle, a feature that made them useful models to …

Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies

L Paturle-Lafanechère, M Manier… - Journal of cell …, 1994 - journals.biologists.com
Tubulin is the major protein component of brain tissue. It normally undergoes a cycle of
tyrosination-detyrosination on the carboxy terminus of its α-subunit and this results in …

Induction of tubulin synthesis during cilia regeneration in growing Tetrahymena

RC Bird, AM Zimmerman - Experimental Cell Research, 1980 - Elsevier
Log growth Tetrahymena pyriformis GL were deciliated by means of a calcium pulse and
allowed to regenerate their cilia in a non-nutrient recovery medium. Polyribosome profiles …

Structural thermal adaptation of β‐tubulins from the Antarctic psychrophilic protozoan Euplotes focardii

F Chiappori, S Pucciarelli, I Merelli… - Proteins: Structure …, 2012 - Wiley Online Library
Tubulin dimers of psychrophilic eukaryotes can polymerize into microtubules at 4° C, a
temperature at which microtubules from mesophiles disassemble. This unique capability …

Shape of the ciliary doublet microtubule in solution

Y Takasaki, T Miki-Noumura - Journal of molecular biology, 1982 - Elsevier
The individual doublet microtubule (DMT) from Tetrahymena cilia had the appearance of a
circular arc in solution and its contour length was around 5 to 6 μm as observed under a …