A single medium supports development of bovine embryos throughout maturation, fertilization and culture

AP Gandhi, M Lane, DK Gardner… - Human …, 2000 - academic.oup.com
Oocytes and embryos are typically exposed sequentially to varying culture media in
standard in-vitro protocols. Expenditures of energy may be required following each medium …

Effects of co-culture and embryo number on the in vitro development of bovine embryos

I Donnay, A Van Langendonckt, P Auquier, B Grisart… - Theriogenology, 1997 - Elsevier
It is generally accepted that culturing embryos in groups or with somatic cells improves both
the yield and quality of the blastocysts obtained. The aims of this study were 1) to compare …

Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media

BD Bavister, TA Rose-Hellekant, T Pinyopummintr - Theriogenology, 1992 - Elsevier
The techniques of in vitro maturation (IVM) and in vitro fertilization (IVF) of bovine oocytes,
with development of the resulting embryos to advanced preimplantation stages in vitro, have …

Simple and efficient chemically defined in vitro maturation and embryo culture system for bovine embryos

ME Arias, T Vargas, V Gallardo, L Aguila, R Felmer - Animals, 2022 - mdpi.com
Simple Summary Supplementation of the culture media for the in vitro production of bovine
embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. This study …

In vitro developmental competence of in vitro-matured bovine oocytes fertilized and cultured in completely defined media

L Keskintepe, BG Brackett - Biology of Reproduction, 1996 - academic.oup.com
The objective was to establish an in vitro system in which bovine oocytes can be matured,
fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic …

Development of bovine oocytes matured, fertilized and cultured in a serum-free, chemically defined medium

Y Takagi, K Mori, M Tomizawa, T Takahashi… - Theriogenology, 1991 - Elsevier
This experiment was conducted to determine if bovine embryos derived from in vitro
maturation, fertilization and culture could develop in serum-free medium. Oocytes were …

Production of viable bovine blastocysts in defined in vitro conditions.

L Keskintepe, CA Burnley, BG Brackett - Biology of reproduction, 1995 - europepmc.org
Our purpose was to obtain viable blastocysts via in vitro maturation, fertilization, and culture
(IVMFC) in serum-and BSA-free media (defined conditions) and to document viability by …

Development of bovine embryos in a cell-free culture medium: effects of type of serum, timing of its inclusion and heat inactivation

T Pinyopummintr, BD Bavister - Theriogenology, 1994 - Elsevier
Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to
investigate the effects of timing of serum inclusion in the culture medium and different types …

In vitro-matured/in vitro-fertilized bovine oocytes can develop into morulae/blastocysts in chemically defined, protein-free culture media

T Pinyopummintr, BD Bavister - Biology of reproduction, 1991 - academic.oup.com
Abstract Development of bovine embryos derived from in vitro-matured/in vitro-fertilized
(IVM/IVF) oocytes was examined in two culture media: hamster embryo culture medium …

Current status of IVM/IVF and embryo culture in humans and farm animals

A Trounson, D Pushett, LJ Maclellan, I Lewis… - Theriogenology, 1994 - Elsevier
Human oocytes are usually recovered from preovulatory follicles of women treated with
gonadotropins and gonadotropin releasing hormone analogue. The oocytes are usually …