The generation of non-thienamycin-producing mutants with mutations in the thnL, thnN,
thnO, and thnI genes within the thn gene cluster from Streptomyces cattleya and their …

Two regulatory genes, thnI and thnU, were identified in the thienamycin (thn) gene cluster
from Streptomyces cattleya. ThnI resembles LysR‐type transcriptional activators and ThnU …

In silico database searches allowed the identification in the S. flavogriseus ATCC 33331
genome of a carbapenem gene cluster highly related to the S. cattleya thienamycin one …

The enzymatic activities of three proteins encoded by the thienamycin gene cluster of
Streptomyces cattleya (ThnR, ThnH, and ThnT) have been shown to incrementally cleave …

Isolation, cloning and Sequencing of the cluster of genes involved in the biosynthesis of the
carbapenem thienamycin by Streptomyces cattleya, and the use of those genes to increase …

Materials and Methods Characterization of Mutants and Cross-Feeding Assays
Streptomyces cattleya MA4297wasmutagenized with EMS (ethyl methanesulfonate) …

II-- I, III, XVI III I, IV XVI XV-- III, XVI XVI I, II, III, XV--5953 to produce THM. The cosynthetic
bioassay involves adding the bioconvertible material of MA 5952 to the resting cells of MA …

Abstract β-lactam ring formation in carbapenem and clavam biosynthesis proceeds through
an alternative mechanism to the biosynthetic pathway of classic β-lactam antibiotics. This …

A gene, pcbC, encoding the isopenicillin N synthase of Streptomyces griseus NRRL 3851,
has been cloned in a 6.4-kb Bg/II DNA fragment and located in an internal 1.55-kb PvuII …

Streptomyces tendae Tü901 produces nikkomycins belonging to the nucleoside peptide
antibiotics. Mutants defective in histidine catabolism were isolated and characterized with …