A Rev-Independent Human Immunodeficiency Virus Type 1 (HIV-1)-Based Vector That Exploits a Codon-Optimized HIV-1gag-pol Gene
E Kotsopoulou, VN Kim, AJ Kingsman… - Journal of …, 2000 - Am Soc Microbiol
The human immunodeficiency virus (HIV) genome is AU rich, and this imparts a codon bias
that is quite different from the one used by human genes. The codon usage is particularly …
that is quite different from the one used by human genes. The codon usage is particularly …
Rev-Independent Expression of Synthetic gag-pol Genes of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus: Implications for the Safety of …
R Wagner, M Graf, K Bieler, H Wolf, T Grunwald… - Human gene …, 2000 - liebertpub.com
The safety of lentiviral vectors for clinical applications is still a major concern. The gag-pol
expression plasmids and the lentiviral vectors used in previous studies contain homologous …
expression plasmids and the lentiviral vectors used in previous studies contain homologous …
Continuous high-titer HIV-1 vector production
Human immunodeficiency virus type 1 (HIV-1)–based vectors are currently made by
transient transfection, or using packaging cell lines in which expression of HIV-1 Gag and …
transient transfection, or using packaging cell lines in which expression of HIV-1 Gag and …
Human immunodeficiency virus type 1 Pr55gag and Pr160gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled …
AJ Smith, MI Cho, ML Hammarskjöld… - Journal of virology, 1990 - Am Soc Microbiol
Human immunodeficiency virus type 1 (HIV-1) gag and pol genes were expressed by using
fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector …
fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector …
HIV-1 regulator of virion expression (Rev) protein binds to an RNA stem-loop structure located within the Rev response element region
S Heaphy, C Dingwall, I Ernberg, MJ Gait, SM Green… - Cell, 1990 - cell.com
HIV-1 Rev protein, purified from E. coli, binds specifically to an RNA transcript containing the
223 nucleotide long Rev response element (RRE) sequence. Rev binds to RRE in vitro with …
223 nucleotide long Rev response element (RRE) sequence. Rev binds to RRE in vitro with …
Mutational inactivation of an inhibitory sequence in human immunodeficiency virus type 1 results in Rev-independent gag expression
S Schwartz, M Campbell, G Nasioulas… - Journal of …, 1992 - Am Soc Microbiol
We have characterized an inhibitory RNA element in the human immunodeficiency virus
type 1 (HIV-1) gag coding sequence that prevents gag expression. The inhibition exerted by …
type 1 (HIV-1) gag coding sequence that prevents gag expression. The inhibition exerted by …
Inactivation of the human immunodeficiency virus type 1 inhibitory elements allows Rev-independent expression of Gag and Gag/protease and particle formation
R Schneider, M Campbell, G Nasioulas… - Journal of …, 1997 - Am Soc Microbiol
The expression of gag, pol, and env of human immunodeficiency virus type 1 (HIV-1)
depends on the presence of the viral Rev protein. This dependence is, at least in part, due to …
depends on the presence of the viral Rev protein. This dependence is, at least in part, due to …
rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.
BK Felber, M Hadzopoulou-Cladaras… - Proceedings of the …, 1989 - National Acad Sciences
rev (trs/art) is an essential human immunodeficiency virus type 1 (HIV-1) regulatory protein.
rev increases the levels of the gag-and env-producing mRNAs via a cis-acting element in …
rev increases the levels of the gag-and env-producing mRNAs via a cis-acting element in …
Elements distinct from human immunodeficiency virus type 1 splice sites are responsible for the Rev dependence of env mRNA
G Nasioulas, AS Zolotukhin, C Tabernero… - Journal of …, 1994 - Am Soc Microbiol
In the absence of the viral regulatory protein Rev, the human immunodeficiency virus type 1
gag/pol and env mRNAs are inefficiently expressed, since nucleocytoplasmic transport …
gag/pol and env mRNAs are inefficiently expressed, since nucleocytoplasmic transport …
Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decrease RNA stability and inhibit expression in the absence of Rev protein
S Schwartz, BK Felber, GN Pavlakis - Journal of virology, 1992 - Am Soc Microbiol
The expression of Gag, Pol, Vif, Vpr, Vpu, and Env proteins from unspliced and partially
spliced human immunodeficiency virus type 1 (HIV-1) mRNAs depends on the viral protein …
spliced human immunodeficiency virus type 1 (HIV-1) mRNAs depends on the viral protein …