The assessment of drug-dose responses is vital for the prediction of unwanted toxicological
effects in modern medicine. Three-dimensional (3D) cell cultures techniques can provide in …

A recent decline in the discovery of novel medications challenges the widespread use of 2D
monolayer cell assays in the drug discovery process. As a result, the need for more …

Purpose: To give a state-of-the-art overview on the promise of three-dimensional (3-D)
culture systems for anticancer drug development, with particular emphasis on multicellular …

Drug resistance remains a major clinical problem despite advances in targeted therapies. In
recent years, methods to culture cancer cells in three-dimensional (3D) environments to …

Clinically relevant in vitro methods are needed to identify new cancer drugs for solid tumors.
We report on a new 3-D spheroid cell culture system aimed to mimic the properties of solid …

The potential of a spheroid tumor model composed of cells in different proliferative and
metabolic states for the development of new anticancer strategies has been amply …

Tumor spheroid models have proven useful in the study of cancer cell responses to
chemotherapeutic compounds by more closely mimicking the 3-dimensional nature of …

Introduction: Drug screening assays employing two-dimensional (2D) cultures of cancer
cells have been largely replaced by three-dimensional (3D) multicellular tumor spheroid …

Spheroids present a biologically relevant three-dimensional model of avascular tumors and
a unique tool for discovery of anticancer drugs. Despite being used in research laboratories …

There is an increasing interest in using three-dimensional (3D) spheroids for modeling
cancer and tissue biology to accelerate translation research. Development of higher …