Expanding the range of CRISPR/Cas9 genome editing in rice

X Hu, C Wang, Y Fu, Q Liu, X Jiao, K Wang - Molecular plant, 2016 - cell.com
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …

Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice

S Li, X Zhang, W Wang, X Guo, Z Wu, W Du, Y Zhao… - Molecular Plant, 2018 - cell.com
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

[HTML][HTML] xCas9 expands the scope of genome editing with reduced efficiency in rice

J Wang, X Meng, X Hu, T Sun, J Li… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
CRISPR-Cas9 systems have been widely used in animals and plants for genome editing,
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …

Hypercompact CRISPR–Cas12j2 (CasΦ) enables genome editing, gene activation, and epigenome editing in plants

S Liu, S Sretenovic, T Fan, Y Cheng, G Li, A Qi… - Plant …, 2022 - cell.com
CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …

Recent advances in genome editing using CRISPR/Cas9

Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …

The ScCas9++ variant expands the CRISPR toolbox for genome editing in plants

T Liu, D Zeng, Z Zheng, Z Lin, Y Xue… - Journal of integrative …, 2021 - Wiley Online Library
The development of clustered regularly interspaced palindromic repeats (CRISPR)‐
associated protein (Cas) variants with a broader recognition scope is critical for further …

Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system

J Li, Y Sun, J Du, Y Zhao, L Xia - Molecular plant, 2017 - cell.com
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-
associated Cas9 endonuclease)-mediated genome editing has revolutionized biological …

[PDF][PDF] Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice

X Meng, X Hu, Q Liu, X Song, C Gao… - Science China. Life …, 2018 - gaolab.genetics.ac.cn
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) system
has been widely used for a variety of applications, including targeted gene knockout, gene …

Multiplex gene editing in rice using the CRISPR-Cpf1 system

M Wang, Y Mao, Y Lu, X Tao, J Zhu - Molecular plant, 2017 - cell.com
The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9
system has been used successfully for simultaneous modification of multiple loci in plants …