Difficulty in gene transduction of human blood cells, including hematopoietic stem cells, has
hampered the development of gene therapy applications for hematological disorders …

Lentivirus vectors can transduce dividing and nondividing cells. Using three-plasmid
transient transfections, high-titer (> 109 IU/ml) recombinant lentivirus vectors pseudotyped …

The ultimate success of gene therapy to cure inherited or acquired genetic diseases relies
on the development and on the availability of gene transfer vectors that can efficiently deliver …

Recently, gene delivery vectors based on human immunodeficiency virus (HIV) have been
developed as an alternative mode of gene delivery. These vectors have a number of …

In addition to the long-term expression of the transgene provided by all retroviral vectors,
lentiviruses present the opportunity to transduce nondividing cells and potentially achieve …

Background HIV-responsive expression vectors are all based on the HIV promoter, the long
terminal repeat (LTR). While responsive to an early HIV protein, Tat, the LTR is also …

Previously we described safe and efficient three-component human immunodeficiency virus
type 1 (HIV-1)-based gene transfer systems for delivery of genes into nondividing cells (H …

HIV-1-Derived Lentiviral Vectors Page 1 HIV-1-Derived Lentiviral Vectors LE AILLES and L.
NALDINI 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 2 HIV-1 …

ABSTRACT A number of human immunodeficiency type 1 (HIV-1)-based vectors have
recently been shown to transduce nondividing cells in vivo as well as in vitro. However, if …

Despite the ability to infect nonproliferating cells, current lentiviral vectors are inefficient at
mediating gene transfer into quiescent primary human cells such as monocytes. Here, a …