Decades of work have aimed to genetically reprogram T cells for therapeutic purposes,
using recombinant viral vectors, which do not target transgenes to specific genomic sites …

CRISPR/Cas9 technologies have revolutionized our understanding of gene function in
complex biological settings, including T cell immunology. Current CRISPR-mediated gene …

Therapies that target the function of immune cells have significant clinical efficacy in
diseases such as cancer and autoimmunity. Although functional genomics has accelerated …

The advent of engineered T cells as a form of immunotherapy marks the beginning of a new
era in medicine, providing a transformative way to combat complex diseases such as …

CRISPR-enabled screening is a powerful tool for the discovery of genes that control T cell
function and has nominated candidate targets for immunotherapies,,,,–. However, new …

Gene editing can be used to overcome allo-recognition, which otherwise limits allogeneic T
cell therapies. Initial proof-of-concept applications have included generation of such" …

Chimeric antigen receptor (CAR) T cells have shown great promise in the treatment of
hematological and solid malignancies. However, despite the success of this field, there …

T cell receptors (TCRs) enable T cells to specifically recognize mutations in cancer cells,–.
Here we developed a clinical-grade approach based on CRISPR–Cas9 non-viral precision …

T-cell genome engineering holds great promise for cell-based therapies for cancer, HIV,
primary immune deficiencies, and autoimmune diseases, but genetic manipulation of human …

Multiple clinical trials of allogeneic T cell therapy use site-specific nucleases to disrupt T cell
receptor (TCR) and other genes,,,,–. In this study, using single-cell RNA sequencing, we …