CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation
The CRISPR/Cas9 system was recently developed as a powerful and flexible technology for
targeted genome engineering, including genome editing (altering the genetic sequence) …
targeted genome engineering, including genome editing (altering the genetic sequence) …
Updates on gene editing and its applications
Recent advancements in gene editing provide unprecedented tools for plant biology
research and offer unlimited potential for improving existing crops and de novo …
research and offer unlimited potential for improving existing crops and de novo …
Expanding the CRISPR toolbox in zebrafish for studying development and disease
The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …
underlying normal development, adult homeostasis, and human disease. Much of what we …
Generalizable sgRNA design for improved CRISPR/Cas9 editing efficiency
Motivation The development of clustered regularly interspaced short palindromic repeat
(CRISPR)/CRISPR-associated protein 9 (Cas9) technology has provided a simple yet …
(CRISPR)/CRISPR-associated protein 9 (Cas9) technology has provided a simple yet …
Expanding plant genome-editing scope by an engineered iSpyMacCas9 system that targets A-rich PAM sequences
The most popular CRISPR-SpCas9 system recognizes canonical NGG protospacer adjacent
motifs (PAMs). Previously engineered SpCas9 variants, such as Cas9-NG, favor G-rich …
motifs (PAMs). Previously engineered SpCas9 variants, such as Cas9-NG, favor G-rich …
Editing plant genomes with CRISPR/Cas9
K Belhaj, A Chaparro-Garcia, S Kamoun… - Current opinion in …, 2015 - Elsevier
Highlights•Cas9 is an RNA-guided DNA endonuclease innate to prokaryotic immune
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
Targeted gene manipulation in plants using the CRISPR/Cas technology
D Zhang, Z Li, JF Li - Journal of Genetics and Genomics, 2016 - Elsevier
The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …
organisms including plants, and has quickly evolved into a suite of versatile tools for …
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
C Gaillochet, A Peña Fernández, V Goossens… - Genome biology, 2023 - Springer
Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …
when developing new genome engineering tools. Plant biotechnology has few high …
Genome editing in animals with minimal PAM CRISPR-Cas9 enzymes
J Vicencio, C Sánchez-Bolaños… - Nature …, 2022 - nature.com
The requirement for Cas nucleases to recognize a specific PAM is a major restriction for
genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs …
genome editing. SpCas9 variants SpG and SpRY, recognizing NGN and NRN PAMs …