Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …

Genome editing technologies hold tremendous potential in biomedical research and drug
development. Therefore, it is imperative to discover gene editing tools with superior cutting …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

The targeting range of CRISPR–Cas9 base editors (BEs) is limited by their G/C-rich
protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a …

Abstract CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-
CRISPR associated) has been extensively exploited as a genetic tool for genome editing …

The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary
to the guide RNA and has been applied to programmable genome editing. Cas9-mediated …

Abstract The RNA-guided CRISPR/Cas9 system is a powerful tool for genome editing, but its
targeting scope is limited by the protospacer-adjacent motif (PAM). To expand the target …

CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While
the rigid identification of DNA sequences by these systems minimizes the potential for off …

The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

Site-directed RNA editing approaches offer great potential to correct genetic mutations in
somatic cells while avoiding permanent off-target genomic edits. Nuclease-dead RNA …