The class 2 CRISPR-Cas endonuclease Cas12a (previously known as Cpf1) offers several
advantages over Cas9, including the ability to process its own array and the requirement for …

The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-
associated nuclease 9) method has been dramatically changing the field of genome …

The popularity of CRISPR-based gene editing has resulted in an abundance of tools to
design CRISPR-Cas9 guides. This is also driven by the fact that designing highly specific …

Success with genome editing by the RNA-programmed nuclease Cas9 has been limited by
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …

Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an
alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR …

CRISPR prime editing (PE) requires a Cas9 nickase-reverse transcriptase fusion protein
(known as PE2) and a prime editing guide RNA (pegRNA), an extended version of a …

Highlights●Adapting new nucleases and improving established ones expand the
CRISPR/Cas toolbox.●Optimized base editors enlarge editing windows and enable CG …

CRISPR-Cas9 has emerged as a revolutionary tool that enables precise and efficient
modifications of the genetic material. This review provides a comprehensive overview of …

Existing adenine and cytosine base editors induce only a single type of modification, limiting
the range of DNA alterations that can be created. Here we describe a CRISPR–Cas9-based …

CRISPR effector proteins introduce double-stranded breaks into the mammalian genome,
facilitating gene editing by non-homologous end-joining or homology-directed repair. Unlike …