Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design
B Farboud, BJ Meyer - Genetics, 2015 - academic.oup.com
Success with genome editing by the RNA-programmed nuclease Cas9 has been limited by
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
[HTML][HTML] In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …
the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni …
[HTML][HTML] Web-based design and analysis tools for CRISPR base editing
Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has
been widely used as a genome editing tool. Recently, CRISPR base editors, which consist …
been widely used as a genome editing tool. Recently, CRISPR base editors, which consist …
Enhancement of target specificity of CRISPR–Cas12a by using a chimeric DNA–RNA guide
H Kim, W Lee, Y Oh, SH Kang, JK Hur… - Nucleic acids …, 2020 - academic.oup.com
The CRISPR–Cas9 system is widely used for target-specific genome engineering. CRISPR–
Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing …
Cas12a (Cpf1) is one of the CRISPR effectors that controls target genes by recognizing …
[HTML][HTML] Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity
H Huang, G Huang, Z Tan, Y Hu, L Shan, J Zhou… - BMC biology, 2022 - Springer
Abstract Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-
editing tool with properties distinct from the broadly used Cas9 system. Features such as …
editing tool with properties distinct from the broadly used Cas9 system. Features such as …
[HTML][HTML] Computational approaches for effective CRISPR guide RNA design and evaluation
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-
associated (Cas) system has emerged as the main technology for gene editing. Successful …
associated (Cas) system has emerged as the main technology for gene editing. Successful …
Potential pitfalls of CRISPR/Cas9‐mediated genome editing
R Peng, G Lin, J Li - The FEBS journal, 2016 - Wiley Online Library
Recently, a novel technique named the clustered regularly interspaced short palindromic
repeat (CRISPR)/CRISPR‐associated protein (Cas) 9 system has been rapidly developed …
repeat (CRISPR)/CRISPR‐associated protein (Cas) 9 system has been rapidly developed …
[HTML][HTML] Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing
Compact and versatile CRISPR-Cas systems will enable genome engineering applications
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens
D Peng, R Tarleton - Microbial genomics, 2015 - microbiologyresearch.org
Recent development of CRISPR-Cas9 genome editing has enabled highly efficient and
versatile manipulation of a variety of organisms and adaptation of the CRISPR-Cas9 system …
versatile manipulation of a variety of organisms and adaptation of the CRISPR-Cas9 system …
[HTML][HTML] Technologies and computational analysis strategies for CRISPR applications
The CRISPR-Cas system offers a programmable platform for eukaryotic genome and
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …