[HTML][HTML] Computational approaches for effective CRISPR guide RNA design and evaluation
Abstract The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-
associated (Cas) system has emerged as the main technology for gene editing. Successful …
associated (Cas) system has emerged as the main technology for gene editing. Successful …
Technologies and computational analysis strategies for CRISPR applications
The CRISPR-Cas system offers a programmable platform for eukaryotic genome and
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing
Compact and versatile CRISPR-Cas systems will enable genome engineering applications
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
[HTML][HTML] Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0
CRISPR/Cas9 systems are an established tool in genome engineering. As double strand
breaks caused by the standard Cas9-based knock-out techniques can be problematic in …
breaks caused by the standard Cas9-based knock-out techniques can be problematic in …
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
CRISPR–Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that
partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) …
partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) …
CRISPRon/off: CRISPR/Cas9 on-and off-target gRNA design
C Anthon, GI Corsi, J Gorodkin - Bioinformatics, 2022 - academic.oup.com
The effectiveness of CRISPR/Cas9-mediated genome editing experiments largely depends
on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and …
on the guide RNA (gRNA) used by the CRISPR/Cas9 system for target recognition and …
Evolution of CRISPR/cas systems for precise genome editing
M Hryhorowicz, D Lipiński, J Zeyland - International Journal of Molecular …, 2023 - mdpi.com
The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
A modular cloning toolkit for genome editing in plants
Background CRISPR/Cas has recently become a widely used genome editing tool in
various organisms, including plants. Applying CRISPR/Cas often requires delivering …
various organisms, including plants. Applying CRISPR/Cas often requires delivering …
Multiplexed conditional genome editing with Cas12a in Drosophila
F Port, M Starostecka… - Proceedings of the …, 2020 - National Acad Sciences
CRISPR-Cas genome engineering has revolutionized biomedical research by enabling
targeted genome modification with unprecedented ease. In the popular model organism …
targeted genome modification with unprecedented ease. In the popular model organism …
Targeted plant genome editing via the CRISPR/Cas9 technology
Targeted modification of plant genome is key for elucidating and manipulating gene
functions in basic and applied plant research. The CRISPR (clustered regularly interspaced …
functions in basic and applied plant research. The CRISPR (clustered regularly interspaced …