Comparison of predictive methods and biological validation for qPCR reference genes in sunflower leaf senescence transcript analysis

P Fernandez, JA Di Rienzo, S Moschen, GAA Dosio… - Plant cell reports, 2011 - Springer
The selection and validation of reference genes constitute a key point for gene expression
analysis based on qPCR, requiring efficient normalization approaches. In this work, the …

[HTML][HTML] Selection of reference genes for qPCR-and ddPCR-based analyses of gene expression in senescing barley leaves

A Zmienko, A Samelak-Czajka, M Goralski… - PloS one, 2015 - journals.plos.org
Leaf senescence is a tightly regulated developmental or stress-induced process. It is
accompanied by dramatic changes in cell metabolism and structure, eventually leading to …

[HTML][HTML] Selection of Reference Genes for Quantitative Gene Expression Studies in Platycladus orientalis (Cupressaceae) Using Real-Time PCR

E Chang, S Shi, J Liu, T Cheng, L Xue, X Yang… - PloS one, 2012 - journals.plos.org
Platycladus orientalis is a tree species that is highly resistant, widely adaptable, and long-
lived, with lifespans of even thousands of years. To explore the mechanisms underlying …

Validation of reliability for reference genes under various abiotic stresses in tea plant

QP Ma, S Hao, X Chen, XH Li - Russian Journal of Plant Physiology, 2016 - Springer
Reference genes are frequently used as a normalization standard to obtain reliable data
during quantitative real-time polymerase chain reaction (qRT-PCR). However, recent studies …

[HTML][HTML] Validation of reference genes for gene expression studies in post-harvest leaves of tea plant (Camellia sinensis)

Z Zhou, H Deng, Q Wu, B Liu, C Yue, T Deng, Z Lai… - PeerJ, 2019 - peerj.com
Tea is one of three major non-alcoholic beverages that are popular all around the world. The
economic value of tea product largely depends on the post-harvest physiology of tea leaves …

[HTML][HTML] Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves

C Tian, Q Jiang, F Wang, GL Wang, ZS Xu, AS Xiong - PLoS one, 2015 - journals.plos.org
Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops
in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A …

[HTML][HTML] Identification and validation of reference genes for gene expression studies in postharvest rose flower (Rosa hybrida)

Y Meng, N Li, J Tian, J Gao, C Zhang - Scientia Horticulturae, 2013 - Elsevier
Optimal reference genes are important for data normalization so that accurate and reliable
gene expression measurements may be obtained in both semi-quantitative reverse …

[HTML][HTML] Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis of Metasequoia

JJ Wang, S Han, W Yin, X Xia, C Liu - International Journal of Molecular …, 2018 - mdpi.com
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most
sensitive technique for evaluating gene expression levels. Choosing appropriate reference …

[HTML][HTML] Identification of stable reference genes for qPCR studies in common wheat (Triticum aestivum L.) seedlings under short-term drought stress

K Dudziak, M Sozoniuk, H Szczerba, A Kuzdraliński… - Plant Methods, 2020 - Springer
Abstract Background Quantitative PCR (qPCR) is one of the most common and accurate
methods of gene expression analysis. However, the biggest challenge for this kind of …

Systematic selection and validation of appropriate reference genes for gene expression studies by quantitative real-time PCR in pear

Y Xu, H Li, X Li, J Lin, Z Wang, Q Yang… - Acta physiologiae …, 2015 - Springer
RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …