Time-domain fluorescence lifetime imaging applied to biological tissue

D Elson, J Requejo-Isidro, I Munro, F Reavell… - Photochemical & …, 2004 - Springer
Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

Fluorescence lifetime imaging–techniques and applications

W Becker - Journal of microscopy, 2012 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Fluorescence lifetime imaging microscopy (FLIM)

EB van Munster, TWJ Gadella - Microscopy Techniques: -/-, 2005 - Springer
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial
distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles

J Siegel, DS Elson, SED Webb, KCB Lee, A Vlandas… - Applied …, 2003 - opg.optica.org
We have applied fluorescence lifetime imaging (FLIM) to the autofluorescence of different
kinds of biological tissue in vitro, including animal tissue sections and knee joints as well as …

Time‐domain whole‐field fluorescence lifetime imaging with optical sectioning

MJ Cole, J Siegel, SED Webb, R Jones… - Journal of …, 2001 - Wiley Online Library
A whole‐field time‐domain fluorescence lifetime imaging (FLIM) microscope with the
capability to perform optical sectioning is described. The excitation source is a mode‐locked …

Fluorescence lifetime imaging microscopy for quantitative biological imaging

LC Chen, WR Lloyd III, CW Chang, D Sud… - Methods in cell biology, 2013 - Elsevier
Fluorescence lifetime imaging microscopy (FLIM) is a method for measuring fluorophore
lifetimes with microscopic spatial resolution, providing a useful tool for cell biologists to …

High-speed multispectral fluorescence lifetime imaging implementation for in vivo applications

S Shrestha, BE Applegate, J Park, X Xiao, P Pande… - Optics Letters, 2010 - opg.optica.org
Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for
characterizing the biochemical composition of biological tissue. In recent years, there has …

Recent innovations in fluorescence lifetime imaging microscopy for biology and medicine

R Datta, A Gillette, M Stefely… - Journal of Biomedical …, 2021 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) measures the decay rate of
fluorophores, thus providing insights into molecular interactions. FLIM is a powerful …

Fast fluorescence lifetime imaging techniques: A review on challenge and development

X Liu, D Lin, W Becker, J Niu, B Yu, L Liu… - Journal of Innovative …, 2019 - World Scientific
Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …