The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or
Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and …

Targeted genome editing using engineered nucleases has rapidly transformed from a niche
technology to a mainstream method used by many biological researchers. This widespread …

Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced
short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

Robust and cost-effective genome editing in a diverse array of cells and model organisms is
now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas …

Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing
technology to functional genetics and human therapeutic applications. Here, we show that …