Abstract The Electron Microscopy Data Bank (EMDB) is the global public archive of three-
dimensional electron microscopy (3DEM) maps of biological specimens derived from …

We here introduce the third major release of the SIMPLE (Single-particle IMage Processing
Linux Engine) open-source software package for analysis of cryogenic transmission electron …

Cryo-electron tomography (CET) utilizes a combination of specimen cryo-fixation and multi-
angle electron microscopy imaging to produce three-dimensional (3D) volume …

The MRC binary file format is widely used in the three-dimensional electron microscopy field
for storing image and volume data. Files contain a header which describes the kind of data …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

EM is a software package developed for image processing in the field of biological electron
microscopy. After a brief summary of its history, an outline of the package is given, by …

100 kV is investigated as the operating voltage for single-particle electron cryomicroscopy
(cryoEM). Reducing the electron energy from the current standard of 300 or 200 keV offers …

1 Structural biology is in the midst of a revolution. Instrumentation and software
improvements have allowed for the full realization of cryo-electron microscopy (cryo-EM) as …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …