This easy-to-follow manual describes tested procedures used to prepare biological samples
for scanning and transmission electron microscopy, as well as methods for cytochemistry …

Databases have become integral parts of data management, dissemination, and mining in
biology. At the Second Annual Conference on Electron Tomography, held in Amsterdam in …

Although the advent of direct electron detectors (DEDs) and software developments have
enabled the routine use of single-particle cryogenic electron microscopy (cryo-EM) for …

Cryogenic electron tomography (cryo-ET) enables the 3D visualization of biological material
at a previously unseeable scale. Carefully controlled cryogenic specimen preparation …

The physical principles of electron–specimen interaction govern the design of specimen
supports for electron cryomicroscopy (cryo-EM). Supports are constructed to suspend …

We report a fully automated procedure for the optimization and interpretation of
reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as …

Resolution measures in molecular electron microscopy provide means to evaluate quality of
macromolecular structures computed from sets of their two-dimensional (2D) line …

Highlights•The number of structures determined by cryo-EM is continuing to grow
rapidly.•Computational methods are helping resolve conformational heterogeneity.• …

Detector technology plays a pivotal role in high-resolution and high-throughput cryo-EM
structure determination. Compared with the first-generation, single-electron counting direct …

Recent technological advances in cryo electron microscopy (cryo-EM) have led to new
opportunities in the structural biology field. Here we benchmark the performance of two 300 …