The σ54 regulon (sigmulon) of Pseudomonas putida

I Cases, DW Ussery… - Environmental …, 2003 - Wiley Online Library
Summary σ54 is unique among the bacterial sigma factors. Besides not being related in
sequence with the rest of such factors, its mechanism of transcription initiation is completely …

Sigma 54 Levels and Physiological Control of the Pseudomonas putida Pu Promoter

P Jurado, LA Fernández, V de Lorenzo - Journal of bacteriology, 2003 - Am Soc Microbiol
The cellular levels of the alternative sigma factor σ54 of Pseudomonas putida have been
examined in a variety of growth stages and culture conditions with a single-chain Fv …

The Role of Bacterial Enhancer Binding Proteins as Specialized Activators of σ54-Dependent Transcription

M Bush, R Dixon - Microbiology and Molecular Biology Reviews, 2012 - Am Soc Microbiol
Bacterial enhancer binding proteins (bEBPs) are transcriptional activators that assemble as
hexameric rings in their active forms and utilize ATP hydrolysis to remodel the conformation …

Eubacterial sigma-factors

M Wösten - FEMS microbiology reviews, 1998 - academic.oup.com
The initiation of transcription is the most important step for gene regulation in eubacteria. To
initiate transcription, RNA polymerase has to associate with a small protein, known as a σ …

σ54-promoter discrimination and regulation by ppGpp and DksA

LMD Bernardo, LUM Johansson, E Skarfstad… - Journal of Biological …, 2009 - ASBMB
The σ 54-factor controls expression of a variety of genes in response to environmental cues.
Much previous work has implicated the nucleotide alarmone ppGpp and its co-factor DksA in …

Metabolic Context and Possible Physiological Themes of ς54-Dependent Genes in Escherichia coli

L Reitzer, BL Schneider - Microbiology and Molecular Biology …, 2001 - Am Soc Microbiol
SUMMARY ς54 has several features that distinguish it from other sigma factors in
Escherichia coli: it is not homologous to other ς subunits, ς54-dependent expression …

Multiple in vivo roles for the− 12-region elements of sigma 54 promoters

L Wang, JD Gralla - Journal of bacteriology, 1998 - Am Soc Microbiol
Alignment of sigma 54-dependent promoters indicates conservation of two sequence
elements. Six nucleotides in the downstream− 12 element were mutated individually to each …

Signal sensing by σ54‐dependent regulators: derepression as a control mechanism

V Shingler - Molecular microbiology, 1996 - Wiley Online Library
Transcription by RNA polymerase utilizing the alternative sigma factor σ54 is regulated by a
distinct class of positive activators designated the σ54‐dependent family. The activities of …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …

Mutational analysis of Escherichia coli σ28 and its target promoters reveals recognition of a composite −10 region, comprised of an 'extended −10' motif and a …

BM Koo, VA Rhodius, EA Campbell… - Molecular …, 2009 - Wiley Online Library
Summary σ28 controls the expression of flagella‐related genes and is the most widely
distributed alternative σ factor, present in motile Gram‐positive and Gram‐negative bacteria …