Bacterial conjugation is the process by which a single strand of a conjugative plasmid is
transferred from donor to recipient. For F plasmid, TraI, a relaxase or nickase, binds a single …

F plasmid conjugative transfer is initiated by the introduction of a site-and strand-specific
nick within the plasmid origin of transfer (oriT). Genetic studies have shown nick formation to …

Cleavage at the F plasmid nic site within the origin of transfer (oriT) requires the F-encoded
proteins TraY and TraI and the host-encoded protein integration host factor in vitro. We …

A central step in the transfer of genetic information during bacterial conjugation of the
Escherichia coli F plasmid involves the formation of a protein-DNA complex, called the …

The DNA transfer stage of conjugation requires the products of the F sex factor genes
traMYDIZ and the cis-acting site oriT. Previous interpretation of genetic and protein analyses …

Bacteria commonly exchange genetic information by the horizontal transfer of conjugative
plasmids. In gram-negative conjugation, a relaxase enzyme is absolutely required to …

The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein
required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally …

The product of the Escherichia coli F plasmid traI gene is required for DNA transfer via
bacterial conjugation. This bifunctional protein catalyzes the unwinding of duplex DNA and …

F factor TraY, a ribbon-helix-helix DNA-binding protein, performs two roles in bacterial
conjugation. TraY binds the F origin of transfer (oriT) to promote nicking of plasmid DNA …

Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …