Mass spectrometry (MS)-based proteomics is increasingly applied in a quantitative format,
often based on labeling of samples with stable isotopes that are introduced chemically or …

Stable isotope labeling by amino acids in cell culture (SILAC) is a simple, robust, yet
powerful approach in mass spectrometry (MS)-based quantitative proteomics. SILAC labels …

Mass spectrometry based proteomics can routinely identify hundreds of proteins in a single
LC-MS run, and methods have been developed for relative quantitation between …

Classically, experiments aimed at studying changes in protein expression have always
followed a small set of proteins. This focused approach was necessary since tools to …

Stable isotope labeling by amino acids in cell culture (SILAC) has become a popular
labeling strategy for peptide quantitation in proteomics experiments. If the SILAC technology …

Stable isotope labeling with amino acids in cell culture (SILAC) is a simple in vivo labeling
strategy for mass spectrometry-based quantitative proteomics. It relies on the metabolic …

Publisher Summary Analysis by combined liquid chromatographic separation and mass
spectrometry (LC-MS) is rapidly becoming the most popular and effective approach for large …

Stable isotope labeling is widely used to encode and quantify proteins in mass-spectrometry-
based proteomics. We compared metabolic labeling with stable isotope labeling by amino …

We describe a method to accurately quantify human tumor proteomes by combining a
mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell …

Stable isotope labeling by amino acids (SILAC) metabolically encodes cell populations for
protein quantification by mass spectrometry. SILAC was introduced in 2002 and the field of …