A sensitive sandwich enzyme-linked immunosorbent assay (sELISA) for the detection of walnut residues in processed food

Z Qin, M Yu, J Zhao, Y Huang, W Zhu, H Lin… - Journal of Food …, 2023 - Elsevier
Walnut can trigger IgE-mediated food allergy and seriously endangers life quality and health
of walnut allergy patients. The objective of the study was to develop a specific and sensitive …

Reliable enzyme-linked immunosorbent assay for the determination of walnut proteins in processed foods

H Doi, Y Touhata, H Shibata, S Sakai… - Journal of agricultural …, 2008 - ACS Publications
Among food allergens of tree nuts, walnuts are a frequent cause of adverse food reactions in
allergic patients. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the …

Determination of walnut protein in processed foods by enzyme-linked immunosorbent assay: Interlaboratory study

S Sakai, R Adachi, H Akiyama… - Journal of AOAC …, 2010 - academic.oup.com
Because food allergens from tree nuts, including walnuts, are a frequent cause of adverse
food reactions for allergic patients, the labeling of foods containing ingredients derived from …

[HTML][HTML] Sensitive ELISA and lateral flow immunoassay for the detection of walnut traces in processed food and working surfaces

A Civera, C Esteban, L Mata, L Sánchez, P Galan-Malo… - Food Chemistry, 2024 - Elsevier
Walnut represents one of the most allergenic nuts that can be found as a hidden allergen. In
this study, sandwich ELISA and lateral flow immunoassay (LFIA), based on the …

Quantitative indirect ELISA for determination of walnut proteins in foods

J Fang, D Chen, C Chen, F Ge, D Liu, B Han… - Food science and …, 2015 - Springer
An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut
proteins in commercial products was developed. Polyclonal antibodies against soluble …

Development of an indirect competitive immunoassay for walnut protein component in food

H Wang, G Li, Y Wu, F Yuan, Y Chen - Food chemistry, 2014 - Elsevier
Among food allergens, walnut is a frequent cause of adverse food reactions in allergic
patients. In this study, the walnut allergen protein 2S albumin precursor (Jug r 1) cDNA was …

Detection of walnut residues in foods using an enzyme‐linked immunosorbent assay

L Niemann, SL Taylor, SL Hefle - Journal of Food Science, 2009 - Wiley Online Library
Tree nuts, including walnuts, can be responsible for allergic reactions. Food manufacturers
have the responsibility to declare the presence of walnuts on packaged foods even when …

[HTML][HTML] Survey of Commercial Food Products for Detection of Walnut (Juglans regia) by Two ELISA Methods and Real Time PCR

R Madrid, A García-García, P Cabrera, I González… - Foods, 2021 - mdpi.com
Labeling of food allergens in accordance with legal regulations is important to protect the
health of allergic consumers. The requirements for detecting allergens in foods involve …

Multimeric recombinant antibody (scFv) for ELISA detection of allergenic walnut. An alternative to animal antibodies

R Madrid, S de la Cruz, A García-García… - Journal of Food …, 2018 - Elsevier
Walnuts are classified as an important allergenic ingredient that can cause severe reactions
in sensitized individuals. To prevent unintended exposure to products containing walnut …

Discovery, verification, and validation of walnut protein marker peptides using LC-MS approaches

K Kim, Y Kim, H Lee, C Lee, S Kim, S Oh, ZY Park - Food Chemistry, 2023 - Elsevier
A key requirement of liquid chromatography-mass spectrometry (LC-MS)-based allergenic
food protein analysis methods is to use protein marker peptides with good analytical …