Species-specific identification of human adenoviruses by a multiplex PCR assay

W Xu, MC McDonough, DD Erdman - Journal of Clinical …, 2000 - Am Soc Microbiol
ABSTRACT A multiplex PCR assay was developed by using primers to the fiber gene that
could differentiate human adenovirus (Ad) species A through F in a single amplification …

PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera

EM Elnifro, RJ Cooper, PE Klapper… - Journal of Clinical …, 2000 - Am Soc Microbiol
Subgenus identification of adenoviruses is of clinical importance and is as informative as
identification by serotype in most clinical situations. A PCR-based identification of …

Multiplex polymerase chain reaction for subgenus‐specific detection of human adenoviruses in clinical samples

P Pring‐Åkerblom, FE John Trijssenaar… - Journal of medical …, 1999 - Wiley Online Library
The polymerase chain reaction (PCR) has been used previously for the detection and typing
of adenoviruses directly in clinical samples. Since under clinical conditions subgenus …

Rapid subgenus identification of human adenovirus isolates by a general PCR

AH Kidd, M Jonsson, D Garwicz, AE Kajon… - Journal of Clinical …, 1996 - Am Soc Microbiol
In most clinical situations involving adenovirus infection, subgenus (subgroup) identification
of an adenovirus isolate is as informative as a finer identification by serotype. A PCR method …

Rapid typing of human adenoviruses by a general PCR combined with restriction endonuclease analysis

A Allard, BO Albinsson, G Wadell - Journal of clinical microbiology, 2001 - Am Soc Microbiol
We have developed a system for rapid typing of adenoviruses (Ads) based on a combination
of PCR and restriction endonuclease (RE) digestion (PCR-RE digestion). Degenerated …

Type‐specific identification of human adenovirus 3, 7, and 21 by a multiplex PCR assay

WH Xu, DD Erdman - Journal of Medical Virology, 2001 - Wiley Online Library
Abstract Human adenovirus (Ad) serotypes 3, 7, and 21 of DNA cluster B: 1 are often
associated with severe respiratory illness, particularly in infants and young children and, in …

Multiplexed, real-time PCR for quantitative detection of human adenovirus

Z Gu, SW Belzer, CS Gibson… - Journal of clinical …, 2003 - Am Soc Microbiol
Adenovirus infection is becoming increasingly recognized as a cause of morbidity and
mortality in the immunosuppressed patient population. While early detection and …

Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41

N Jothikumar, TL Cromeans, VR Hill, X Lu… - Applied and …, 2005 - Am Soc Microbiol
ABSTRACT A quantitative real-time TaqMan PCR assay for detection of human
adenoviruses (HAdV) was developed using broadly reactive consensus primers and a …

Typing of human adenoviruses in specimens from immunosuppressed patients by PCR-fragment length analysis and real-time quantitative PCR

K Ebner, M Rauch, S Preuner… - Journal of Clinical …, 2006 - Am Soc Microbiol
ABSTRACT Currently, 51 human adenovirus (AdV) serotypes, which are divided into six
species (A to F), are known. AdV infections are a major cause of morbidity and mortality in …

PCR-based detection and typing of human adenoviruses in clinical samples

P Pring-Åkerblom, T Adrian, T Köstler - Research in Virology, 1997 - Elsevier
Since human adenoviruses (Ad) are associated with a variety of diseases, there is need for
a fast and sensitive diagnostic procedure. The polymerase chain reaction (PCR) has been …