DNA polymerase β (pol β) from rat brain, overexpressed in Escherichia coli, was used as a
model to study the factors responsible for substrate specificity [k pol, K d (app) and k pol/K d …

DNA polymerase β (pol β) is the smallest and least complex DNA polymerase. The structure
of the enzyme is well understood, but little is known about its catalytic properties, particularly …

Structures of DNA polymerase (pol) β bound to single-nucleotide gapped DNA had revealed
that the lyase and pol domains form a" doughnut-shaped" structure altering the dNTP …

Pre-steady-state kinetic analysis was used to compare the catalytic properties of DNA
polymerase β (Pol β) for single-base gap-filling and regular duplex DNA synthesis. The rate …

The kinetics of nucleotide incorporation into 24/36-mer primer/template DNA by purified fetal
calf thymus DNA polymerase (pol) δ was examined using steady-state and pre-steady-state …

The specific catalytic roles of two groups of DNA polymerase β active site residues identified
from crystal structures were investigated: residues possibly involved in DNA template …

The kinetic parameters (k pol,) for all possible correct and incorrect pairing between the A, T,
G, and C bases were determined for wild-type (WT) rat DNA polymerase β (pol β) and the …

The catalytic reaction mediated by DNA polymerases is known to require two Mg (II) ions,
one associated with dNTP binding and the other involved in metal ion catalysis of the …

We have used random sequence mutagenesis to generate mutants of DNA polymerase β in
an effort to identify amino acid residues important for function, catalytic efficiency and fidelity …

The first pre-steady-state kinetic analysis of the stereoselectivity of a DNA polymerase, Pol β
from rat brain, toward Rp and Sp isomers of dATPαS, and alteration of the stereoselectivity …