Fertilization and early embryology: Alleviation of the '2-cell block'and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical …

DK Gardner, M Lane - Human Reproduction, 1996 - academic.oup.com
The role of amino acids, ethylenediaminetetraacetic acid (EDTA), transferrin, oxygen,
glucose, glutamine, taurine and ammonium in CF1 mouse zygote development in culture …

[PDF][PDF] Alleviation of the'2-cell block'and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA and physical parameters

DK Gardner, M Lane - Human reproduction, 1996 - researchgate.net
The role of amino acids, ethylenediaminetetraacetic acid (EDTA), transferrin, oxygen,
glucose, glutamine, taurine and ammonium in CFl mouse zygote development in culture …

Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid

RA Fissore, KV Jackson, AA Kiessling - Biology of Reproduction, 1989 - academic.oup.com
Abstract Development of zygotes from a hybrid-inbred (B6D2F1) and two random-bred (CD1
and CF1) strains of mice were compared after culture in several modifications of a simple …

Effect of different concentrations of amino acids in human serum and follicular fluid on the development of one-cell mouse embryos in vitro

T Nakazawa, K Ohashi, M Yamada, S Shinoda… - …, 1997 - rep.bioscientifica.com
As a model for establishing an optimized medium for human in vitro fertilization (IVF),
modified human tubal fluid (HTF) media containing amino acids at concentrations found in …

Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium

JA Lawitts, JD Biggers - Biology of reproduction, 1991 - academic.oup.com
The 2-cell block may be caused by inappropriate concentrations of commonly used
constituents of embryo culture media. Almost all zygotes obtained by fertilizing CF1 ova with …

Differential regulation of mouse embryo development and viability by amino acids

M Lane, DK Gardner - Reproduction, 1997 - rep.bioscientifica.com
The amino acid requirements of the preimplantation mouse embryo in culture changes as
development proceeds from the zygote to the blastocyst stage. Eagle's non-essential amino …

Amino acids and ammonium regulate mouse embryo development in culture

DK Gardner, M Lane - Biology of reproduction, 1993 - academic.oup.com
The regulation of 1-cell mouse embryo development in culture by amino acids was
investigated. When the 20 amino acids in Eagle's medium were present, blastocyst …

Amino acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium

JD Biggers, LK McGinnis, M Raffin - Biology of reproduction, 2000 - academic.oup.com
Abstract Development of outbred CF1 mouse zygotes in vitro was studied in a chemically
defined, protein-free medium both with and without amino acids. The addition of amino acids …

Mammalian embryo culture in the absence of serum or somatic cell support.

DK Gardner - 1994 - cabidigitallibrary.org
In this review, 4 areas of embryo research are discussed and their relevance to mammalian
embryo culture is considered. The areas are:(1) perturbation of embryonic development by …

Effects of glutamine and taurine on preimplantation development and cleavage of mouse embryos in vitro

F Devreker, K Hardy - Biology of reproduction, 1997 - academic.oup.com
Glutamine and taurine are reported to improve the development of mouse embryos in vitro,
and glutamine is a component in CZB and KSOM, media optimized to overcome the 2-cell …