Transcriptional gene silencing in mammalian cells by miRNA mimics that target gene promoters
ST Younger, DR Corey - Nucleic acids research, 2011 - academic.oup.com
Synthetic small duplex RNAs that are fully complementary to gene promoters can silence
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
MicroRNA-directed transcriptional gene silencing in mammalian cells
MicroRNAs (miRNAs) regulate gene expression at the posttranscriptional level in the
cytoplasm, but recent findings suggest additional roles for miRNAs in the nucleus. To …
cytoplasm, but recent findings suggest additional roles for miRNAs in the nucleus. To …
Predicting potential miRNA target sites within gene promoters
Synthetic small duplex RNAs that are complementary to gene promoters can activate or
inhibit target gene expression. The potency and robustness of gene modulation by these …
inhibit target gene expression. The potency and robustness of gene modulation by these …
Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells
Animal cells have recently been shown to express a range of∼ 22 nucleotide noncoding
RNAs termed micro RNAs (miRNAs). Here, we show that the human mir-30 miRNA can be …
RNAs termed micro RNAs (miRNAs). Here, we show that the human mir-30 miRNA can be …
Getting to the root of miRNA-mediated gene silencing
A Eulalio, E Huntzinger, E Izaurralde - Cell, 2008 - cell.com
MicroRNAs are∼ 22 nucleotide-long RNAs that silence gene expression
posttranscriptionally by binding to the 3′ untranslated regions of target mRNAs. Although …
posttranscriptionally by binding to the 3′ untranslated regions of target mRNAs. Although …
A biochemical approach to identifying microRNA targets
Identifying the downstream targets of microRNAs (miRNAs) is essential to understanding
cellular regulatory networks. We devised a direct biochemical method for miRNA target …
cellular regulatory networks. We devised a direct biochemical method for miRNA target …
Gene silencing in vitro and in vivo using intronic microRNAs
SL Lin, SY Ying - MicroRNA protocols, 2006 - Springer
Abstract MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering
with intracellular messenger RNAs (mRNAs) that contain either complete or partial …
with intracellular messenger RNAs (mRNAs) that contain either complete or partial …
LNA-modified oligonucleotides mediate specific inhibition of microRNA function
microRNAs are short, endogenous non-coding RNAs that act as post-transcriptional
modulators of gene expression. Important functions for microRNAs have been found in the …
modulators of gene expression. Important functions for microRNAs have been found in the …
Biological basis for restriction of microRNA targets to the 3′ untranslated region in mammalian mRNAs
MicroRNAs (miRNAs) interact with target sites located in the 3′ untranslated regions (3′
UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to …
UTRs) of mRNAs to downregulate their expression when the appropriate miRNA is bound to …
Passenger strand loading in overexpression experiments using microRNA mimics
MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs
is a central component of basic research. Modulation of gene expression by miRNA gain-of …
is a central component of basic research. Modulation of gene expression by miRNA gain-of …