By using mini-Tn5 transposon mutagenesis, two mutants of Pseudomonas putida ATCC
12633 were isolated which showed a marked increase in their sensitivity to carbon …

The RpoN protein was originally identified in Escherichia coli as a sigma (sigma) factor
essential for the expression of nitrogen regulons. In the present study we cloned the …

The cellular levels of the alternative sigma factor σ54 of Pseudomonas putida have been
examined in a variety of growth stages and culture conditions with a single-chain Fv …

The activity of the toluene-responsive ς54 Pu promoter of the pWW0 TOL plasmid of
Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium …

Summary σ54 is unique among the bacterial sigma factors. Besides not being related in
sequence with the rest of such factors, its mechanism of transcription initiation is completely …

Pseudomonas putida encodes 20 e xtra c ytoplasmic sigma f actors (ECFs). In this study, we
show that one of these ECFs, known as ECF-Pp12 (PP3006), plays a role in tolerance of …

An investigation was made into the role of the ptsO gene in carbon source inhibition of the
Pu promoter belonging to the Pseudomonas putida upper TOL (toluene degradation) …

Pseudomonas putida TOL plasmid pWW0 catabolic genes are clustered into two operons.
The first, the upper operon, is controlled by the xylR regulatory gene, whereas the second …

ABSTRACT A gene homologous to the rpoS gene of Escherichia coli was cloned from a
Pseudomonas putida KT2440 gene bank by complementation of the rpoS-deficient strain E …

A reverse genetics approach was used to clone a pex starvation gene that codes for an 18-
kDa polypeptide, designated PexB. Single-copy pexB-lacZ operon fusions were constructed …