Simple Purification Method for a Vibrio vulnificus Hemolysin by a Hydrophobic Column Chromatography in the Presence of a Detergent

E Oh, Y Tamanoi, A Toyoda, K Usui… - Microbiology and …, 1993 - Wiley Online Library
A Vibrio vulnificus hemolysin (VVH) was purified by two steps of hydrophobic column
chromatography on Phenyl‐Sepharose HP. The first chromatography was carried out at pH …

Variability of Haemolysin(s) Produced by Vibrio vulnficus

K Okada, S Miake, T Moriya… - Journal of general …, 1987 - microbiologyresearch.org
The peptide composition and antigenic cross-reactivity of partially purified and concentrated
haemolysins of 16 strains of Vibrio vulnificus were examined by SDS-PAGE and …

Some Properties of Vibrio vulnificus Hemolysin

S Shinoda, SI Miyoshi, H Yamanaka… - Microbiology and …, 1985 - Wiley Online Library
Some properties of hemolysin produced by Vibrio vulnificus were investigated. The
hemolysin was heat labile, and the hemolytic activity was inhibited by adding cholesterol or …

Susceptibility of erythrocytes from several animal species to Vibrio vulnificus hemolysin

H Yamanaka, S Shimatani, M Tanaka… - FEMS microbiology …, 1989 - academic.oup.com
The hemolytic activity of Vibrio vulnificus hemolysin (VVH) against erythrocytes from several
animal species (sheep, horse, cow, rabbit, chicken) was investigated. VVH was active …

Monoclonal Antibodies against the Haemolysin of Vibrio vulnificus

K OKADA, M MITSUYAMA… - Journal of general …, 1987 - microbiologyresearch.org
The extracellular haemolysin produced by Vibrio vulnificus strain FCC was partially purified
from the culture supernate by sequential ammonium sulphate precipitation, gel filtration with …

Mechanism of Haemolysis by Vibrio vulnificus Haemolysin

H Yamanaka, T Satoh, T Katsu… - Journal of general …, 1987 - microbiologyresearch.org
The haemolytic action of Vibrio vulnificus haemolysin (VVH) was compared to that of
streptolysin O (SLO). Both were cholesterol-binding haemolysins, but differed in the release …

Purification and Characterization of Thermostable Direct Hemolysin of Vibrio parahaemolyticus

J Sakurai, A Matsuzaki, T Miwatani - Infection and immunity, 1973 - Am Soc Microbiol
A thermostable direct hemolysin was purified from culture filtrates of Vibrio
parahaemolyticus. The purified hemolysin gave one precipitation line with the antihemolysin …

Suppression and inactivation of Vibrio vulnificus hemolysin in cirrhotic ascites, a human ex vivo experimental system

MH Choi, RY Park, HY Sun, CM Kim… - FEMS Immunology & …, 2006 - academic.oup.com
To elucidate the mechanisms underlying the in vivo suppression and inactivation of Vibrio
vulnificus hemolysin (VvhA), we used cirrhotic ascites fluid as a human ex vivo experimental …

Sandwich enzyme-linked immunosorbent assay for Vibrio vulnificus hemolysin to detect V. vulnificus in environmental specimens

RW Parker, DH Lewis - Applied and environmental microbiology, 1995 - Am Soc Microbiol
Vibrio vulnificus hemolysin, purified by quantitative isoelectric focusing, was used to prepare
rabbit and goat anti-hemolysin. The resulting antibodies were used as capture and detector …

Purification and characterization of Vibrio vulnificus protease

N Miyoshi, C Shimizu, SI Miyoshi… - Microbiology and …, 1987 - jstage.jst.go.jp
MATERIALS AND METHODS Bacterial strain and culture of the bacteria. V. vulnificus L-180,
a strain isolated from the blood of a septicemic human and provided by Itsuo Azuma, was …