FRET analysis reveals distinct conformations of IN tetramers in the presence of viral DNA or LEDGF/p75
JJ Kessl, M Li, M Ignatov, N Shkriabai… - Nucleic acids …, 2011 - academic.oup.com
A tetramer of HIV-1 integrase (IN) stably associates with the viral DNA ends to form a fully
functional concerted integration intermediate. LEDGF/p75, a key cellular binding partner of …
functional concerted integration intermediate. LEDGF/p75, a key cellular binding partner of …
Hiv dna integration
R Craigie, FD Bushman - Cold Spring …, 2012 - perspectivesinmedicine.cshlp.org
Retroviruses are distinguished from other viruses by two characteristic steps in the viral
replication cycle. The first is reverse transcription, which results in the production of a double …
replication cycle. The first is reverse transcription, which results in the production of a double …
In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state
P Lesbats, M Metifiot, C Calmels… - Nucleic acids …, 2008 - academic.oup.com
Abstract HIV-1 integrase (IN) oligomerization and DNA recognition are crucial steps for the
subsequent events of the integration reaction. Recent advances described the involvement …
subsequent events of the integration reaction. Recent advances described the involvement …
Structural basis for HIV‐1 DNA integration in the human genome, role of the LEDGF/P75 cofactor
F Michel, C Crucifix, F Granger, S Eiler… - The EMBO …, 2009 - embopress.org
Integration of the human immunodeficiency virus (HIV‐1) cDNA into the human genome is
catalysed by integrase. Several studies have shown the importance of the interaction of …
catalysed by integrase. Several studies have shown the importance of the interaction of …
Molecular interactions between HIV-1 integrase and the two viral DNA ends within the synaptic complex that mediates concerted integration
A macromolecular nucleoprotein complex in retrovirus-infected cells, termed the
preintegration complex, is responsible for the concerted integration of linear viral DNA …
preintegration complex, is responsible for the concerted integration of linear viral DNA …
[HTML][HTML] Four-tiered π interaction at the dimeric interface of HIV-1 integrase critical for DNA integration and viral infectivity
HIV-1 integrase (IN) is an essential enzyme for viral infection. Here, we report an extensive π
electron orbital interaction between four amino acids, W132, M178, F181 and F185, located …
electron orbital interaction between four amino acids, W132, M178, F181 and F185, located …
Structural basis for functional tetramerization of lentiviral integrase
Experimental evidence suggests that a tetramer of integrase (IN) is the protagonist of the
concerted strand transfer reaction, whereby both ends of retroviral DNA are inserted into a …
concerted strand transfer reaction, whereby both ends of retroviral DNA are inserted into a …
Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding
Z Zhao, CJ McKee, JJ Kessl, WL Santos… - Journal of Biological …, 2008 - ASBMB
To identify functional contacts between HIV-1 integrase (IN) and its viral DNA substrate, we
devised a new experimental strategy combining the following two methodologies. First …
devised a new experimental strategy combining the following two methodologies. First …
Structure-function analyses unravel distinct effects of allosteric inhibitors of HIV-1 integrase on viral maturation and integration
D Bonnard, E Le Rouzic, S Eiler, C Amadori… - Journal of Biological …, 2018 - ASBMB
Recently, a new class of HIV-1 integrase (IN) inhibitors with a dual mode of action, called IN-
LEDGF/p75 allosteric inhibitors (INLAIs), was described. Designed to interfere with the IN …
LEDGF/p75 allosteric inhibitors (INLAIs), was described. Designed to interfere with the IN …
The HIV-1 integrase monomer induces a specific interaction with LTR DNA for concerted integration
The assembly mechanism for the human immunodeficiency virus type 1 (HIV) synaptic
complex (SC) capable of concerted integration is unknown. Molecular and structural studies …
complex (SC) capable of concerted integration is unknown. Molecular and structural studies …