A peripheral element assembles the compact core structure essential for group I intron self-splicing
M Xiao, T Li, X Yuan, Y Shang, F Wang… - Nucleic acids …, 2005 - academic.oup.com
The presence of non-conserved peripheral elements in all naturally occurring group I introns
underline their importance in ensuring the natural intron function. Recently, we reported that …
underline their importance in ensuring the natural intron function. Recently, we reported that …
Effects of Maturase Binding and Mg2+ Concentration on Group II Intron RNA Folding Investigated by UV Cross-Linking
JW Noah, AM Lambowitz - Biochemistry, 2003 - ACS Publications
The Lactococcus lactis Ll. LtrB group II intron encodes a reverse transcriptase/maturase
(LtrA protein) that promotes RNA splicing by stabilizing the catalytically active RNA structure …
(LtrA protein) that promotes RNA splicing by stabilizing the catalytically active RNA structure …
Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions
O Fedorova, LJ Su, AM Pyle - Methods, 2002 - Elsevier
Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we
present construct designs and protocols that were used to develop a set of kinetic …
present construct designs and protocols that were used to develop a set of kinetic …
Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA
ÅB Birgisdottir, S Johansen - Nucleic Acids Research, 2005 - academic.oup.com
The wide, but scattered distribution of group I introns in nature is a result of two processes;
the vertical inheritance of introns with or without losses, and the occasional transfer of …
the vertical inheritance of introns with or without losses, and the occasional transfer of …
Group II intron ribozymes that cleave DNA and RNA linkages with similar efficiency, and lack contacts with substrate 2′-hydroxyl groups
Background: Group II introns are self-cplicing RNAc that hdve niechanistic siniil, irity to the
spliceosomc coniples involved in messenger RNA splicing in eukCiryotes. Thesc …
spliceosomc coniples involved in messenger RNA splicing in eukCiryotes. Thesc …
[PDF][PDF] Ribozyme catalysis from the major groove of group II intron domain 5
BB Konforti, DL Abramovitz, CM Duarte, A Karpeisky… - Molecular cell, 1998 - cell.com
The most highly conserved nucleotides in D5, an essential active site component of group II
introns, consist of an AGC triad, of which the G is invariant. To understand how this G …
introns, consist of an AGC triad, of which the G is invariant. To understand how this G …
Crystal structure of a self-splicing group I intron with both exons
PL Adams, MR Stahley, AB Kosek, J Wang, SA Strobel - Nature, 2004 - nature.com
The discovery of the RNA self-splicing group I intron provided the first demonstration that not
all enzymes are proteins. Here we report the X-ray crystal structure (3.1-Å resolution) of a …
all enzymes are proteins. Here we report the X-ray crystal structure (3.1-Å resolution) of a …
Domain 5 binds near a highly conserved dinucleotide in the joiner linking domains 2 and 3 of a group II intron
M PODAR, JIN ZHUO, M ZHANG, JS FRANZEN… - Rna, 1998 - cambridge.org
Photocrosslinking has identified the joiner between domains 2 and 3 [J (23)] as folding near
domain 5 (D5), a highly conserved helical substructure of group II introns required for both …
domain 5 (D5), a highly conserved helical substructure of group II introns required for both …
Catalytic site components common to both splicing steps of a group II intron
G Chanfreau, A Jacquier - Science, 1994 - science.org
The splicing of group II introns occurs in two steps involving substrates with different
chemical configurations. The question of whether these two steps are catalyzed by a single …
chemical configurations. The question of whether these two steps are catalyzed by a single …
A general two-metal-ion mechanism for catalytic RNA.
TA Steitz, JA Steitz - … of the National Academy of Sciences, 1993 - National Acad Sciences
A mechanism is proposed for the RNA-catalyzed reactions involved in RNA splicing and
RNase P hydrolysis of precursor tRNA. The mechanism postulates that chemical catalysis is …
RNase P hydrolysis of precursor tRNA. The mechanism postulates that chemical catalysis is …