[HTML][HTML] Screening of CRISPR/Cas9 gRNA for mimicking Powdery Mildew resistant MLO ol-2 mutant

A Prajapati, V Nain - Bioinformation, 2021 - ncbi.nlm.nih.gov
Powdery Mildew (PM) caused by fungal pathogen Oidium neolycopersici (O. neolycopersici)
affects both greenhouse and field-grown tomato production. Resistance to PM disease can …

CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

MI Santillán Martínez, V Bracuto, E Koseoglou… - BMC plant biology, 2020 - Springer
Background The development of CRISPR/Cas9 technology has facilitated targeted
mutagenesis in an efficient and precise way. Previously, RNAi silencing of the susceptibility …

CRISPR-Cas9 mediated mutagenesis of a DMR6 ortholog in tomato confers broad-spectrum disease resistance

D Paula de Toledo Thomazella, Q Brail, D Dahlbeck… - BioRxiv, 2016 - biorxiv.org
Pathogenic microbes are responsible for severe production losses in crops worldwide. The
use of disease resistant crop varieties can be a sustainable approach to meet the food …

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

V Nekrasov, C Wang, J Win, C Lanz, D Weigel… - Scientific reports, 2017 - nature.com
Genome editing has emerged as a technology with a potential to revolutionize plant
breeding. In this study, we report on generating, in less than ten months, Tomelo, a non …

Sequence-specific nucleases as tools for enhancing disease resistance in crops

V Nekrasov - Transgenic Research, 2019 - Springer
Genome editing technologies, such as CRISPR/Cas, have recently become valuable tools
for plant reverse genetics as well as crop improvement, including enhancement of disease …

CRISPR/Cas9-mediated targeted mutagenesis of two homoeoalleles in tobacco confers resistance to powdery mildew

W Xuebo, L Dandan, T Xiaolei, C Changchun, Z Xinyao… - Euphytica, 2023 - Springer
Clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 (CRISPR-
associated protein 9) technology has emerged as an effective tool for crop improvement. In …

Plant and fungal genome editing to enhance plant disease resistance using the CRISPR/Cas9 system

NC Paul, SW Park, H Liu, S Choi, J Ma… - Frontiers in Plant …, 2021 - frontiersin.org
Crop production has been substantially reduced by devastating fungal and oomycete
pathogens, and these pathogens continue to threaten global food security. Although …

Recent progress in CRISPR/Cas9-based genome editing for enhancing plant disease resistance

H Boubakri - Gene, 2023 - Elsevier
Nowadays, agricultural production is strongly affected by both climate change and pathogen
attacks which seriously threaten global food security. For a long time, researchers have …

Harnessing CRISPR/Cas9 for Enhanced Disease Resistance in Hot Peppers: A Comparative Study on CaMLO2-Gene-Editing Efficiency across Six Cultivars

JH Park, H Kim - International Journal of Molecular Sciences, 2023 - mdpi.com
The Capsicum annuum Mildew Locus O (CaMLO2) gene is vital for plant defense responses
against fungal pathogens like powdery mildew, a significant threat to greenhouse pepper …

CRISPR/Cas9-mediated editing of PhMLO1 confers powdery mildew resistance in petunia

J Xu, AH Naing, H Kang, SY Lee, W Li… - Plant Biotechnology …, 2023 - Springer
Abstract Mildew Locus O (MLO), which encodes a seven-transmembrane domain protein, is
responsible for the development of powdery mildew disease symptoms in many plant …