Coulombic forces play an important role in facilitating protein–protein interactions. It has
been demonstrated that a strong electrostatic potential between two interacting proteins …

Protein trans‐splicing catalyzed by split inteins is a powerful technique for assembling a
polypeptide backbone from two separate parts. However, split inteins with robust efficiencies …

The protein trans-splicing (PTS) activity of naturally split inteins has found widespread use in
chemical biology and biotechnology. However, currently used naturally split inteins suffer …

Protein trans-splicing by split inteins holds great potential for the chemical modification and
semisynthesis of proteins. However, the structural requirements of the extein sequences …

Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans.
The chemical mechanism of protein trans-splicing is well-understood and has been …

Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and
biotechnology to carry out traceless and specific protein ligation. However, the external …

Split inteins carry out a naturally occurring process known as protein trans‐splicing, where
two protein fragments bind to form a catalytically competent enzyme, then catalyze their own …

Split inteins associate to trigger protein splicing in trans, a post-translational modification in
which protein sequences fused to the intein pair are ligated together in a traceless manner …

Split intein-mediated protein trans-splicing has found extensive applications in chemical
biology, protein chemistry, and biotechnology. However, an enduring limitation of all well …

Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology
and biotechnology. Herein, we describe the use of a consensus design approach to …