Analysis of muscle mitochondrial function with techniques applicable to needle biopsy samples

K Gohil, RHT Edwards, DA Jones - Clinical Physiology, 1981 - Wiley Online Library
Methods are described for the assessment of mitochondrial function in small (less than 50
mg) samples of human skeletal muscle using spectrophotometric assays to follow the …

Investigation of mitochondrial metabolism in small human skeletal muscle biopsy specimens. Improvement of preparation procedure

JC Fischer, W Ruitenbeek, AM Stadhouders… - Clinica chimica acta, 1985 - Elsevier
A method is presented which allows the investigation of almost the complete mitochondrial
content of small human skeletal muscle biopsy specimens. Thorough mechanical disruption …

Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function

HN Rasmussen, UF Rasmussen - Detection of Mitochondrial Diseases, 1997 - Springer
Mitochondria prepared in small scale from skeletal muscle were studied with respiration
measurements and low temperature spectroscopy. The method of preparation was …

[引用][C] Measurement of cytochromes in human skeletal muscle mitochondria, isolated from fresh and frozen stored muscle specimens

H Bookelman, JMF Trijbels, RCA Sengers… - Biochemical …, 1978 - Elsevier
Mitochondria were isolated from small muscle biopsy specimens, and the cytochrome
content was calculated from the reduced minus oxidized difference spectrum recorded at …

Measurement of ATP production and respiratory chain enzyme activities in mitochondria isolated from small muscle biopsy samples

R Wibom, L Hagenfeldt, U von Döbeln - Analytical biochemistry, 2002 - Elsevier
A set of methods suitable for assessment of respiratory chain function in mitochondria
isolated from 25mg of muscle is described. This set of methods includes determination of the …

[引用][C] Methods for study of normal and abnormal skeletal muscle mitochondria

HSA Sherratt, NJ Watmough… - Methods of …, 1988 - Wiley Online Library
This chapter contains sections titled: Introduction Diagnosis of Muscle Diseases with
Mitochondrial Malfunction Sources and Handling of Skeletal Muscle Cytochemical …

Defects in the mitochondrial energy metabolism outside the respiratory chain and the pyruvate dehydrogenase complex

FJM Trijbels, W Ruitenbeek, M Huizing… - Detection of …, 1997 - Springer
Disturbances in substrate oxidations in muscle mitochondria from patients with a suspicion
of a mitochondrial myopathy may arise from a deficiency of one or more of the complexes of …

High resolution respirometry of permeabilized skeletal muscle fibers in the diagnosis of neuromuscular disorders

W Sperl, D Skladal, E Gnaiger, M Wyss, U Mayr… - Molecular and cellular …, 1997 - Springer
High resolution respirometry in combination with the skinned fiber technique offers the
possibility to study mitochondrial function routinely in small amounts of human muscle …

Biochemical functioning of mitochondria in normal and denervated mammalian skeletal muscle

M Joffe, N Savage, H Isaacs - … & Nerve: Official Journal of the …, 1981 - Wiley Online Library
Mitochondrial preparations derived from denervated rat skeletal muscle and paired control
muscle are characterized with respect to their oxidative and phosphorylative capacities. Our …

[引用][C] Characteristics of skeletal muscle mitochondria isolated by a new, improved technique

JB Peter, LD Lee - Biochemical and Biophysical Research …, 1967 - Elsevier
Relatively few studies of mitochondria isolated from normal and diseased mammalian
skeletal muscle have been reported (Azzone and Carafoli, 1960; Azzone & a& 1961; …