Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis

CH Zhang, XG Mei, L Liu, LJ Yu - Biotechnology Letters, 2000 - Springer
Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium.
Addition of 50 mg chitosan l− 1, 60 μM methyl jasmonate and 30 μM Ag+ resulted in the …

Enhancement of paclitaxel production by abscisic acid in cell suspension cultures of Taxus chinensis

J Luo, L Liu, CD Wu - Biotechnology Letters, 2001 - Springer
Addition of 5 mg abscisic acid l− 1 after 12 days' growth of Taxus chinensis suspension
culture gave the greatest paclitaxel accumulation at 11 mg l− 1, which was almost 5 times …

Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis

CH Zhang, HB Xu - Biotechnology letters, 2001 - Springer
Dibutyl phthalate, oleic acid and terpineol were used to extract paclitaxel in situ from Taxus
chinensis suspension cultures. Oleic acid/terpineol (1: 1, v/v) added to the cultures gave a …

Improved Paclitaxel and Baccatin III Production in Suspension Cultures of Taxusmedia

R M. Cusidó, J Palazón, M Bonfill… - Biotechnology …, 2002 - Wiley Online Library
A cell suspension culture of Taxus media was established from a stable callus line of this
species. The growth rate and production of paclitaxel and baccatin III of this cell suspension …

Optimization of elicitors and precursors for paclitaxel production in cell suspension culture of Taxus chinensis in the presence of nutrient feeding

J Luo, GY He - Process biochemistry, 2004 - Elsevier
Elicitors and precursors were optimized for paclitaxel production in cell cultures of Taxus
chinensis in the presence of nutrient feeding. The effects of nutrients feeding on cell growth …

Effects of inoculum size and age on biomass growth and paclitaxel production of elicitor-treated Taxus yunnanensis cell cultures

CH Zhang, JY Wu, GY He - Applied microbiology and biotechnology, 2002 - Springer
Suspension cultures of Taxus yunnanensis cells were inoculated with cells of different
culture ages (12–24 days) at various densities [50–250 g fresh weight (fw)/l], and treated (on …

Manipulation by culture mixing and elicitation of paclitaxel and baccatin III production in Taxus baccata suspension cultures

M Bonfill, O Exposito, E Moyano, RM Cusidó… - In Vitro Cellular & …, 2006 - Springer
Experiments were carried out with Taxus baccata cell lines showing different paclitaxel-
producing capacities (between 1.74 and 19.91 mgl− 1) when growing in a selected product …

Enhanced paclitaxel productivity and release capacity of Taxus chinensis cell suspension cultures adapted to chitosan

CH Zhang, PS Fevereiro, G He, Z Chen - Plant Science, 2007 - Elsevier
This work characterized the growth and paclitaxel production and extracellular release of
Taxus chinensis cell suspension cultures adapted to chitosan stress by comparing with the …

Kinetic Studies of Paclitaxel Production by Taxus canadensis Cultures in Batch and Semicontinuous with Total Cell Recycle

M Phisalaphong, JC Linden - Biotechnology progress, 1999 - Wiley Online Library
Suspension cultures of Taxus canadensis were elicited with methyl jasmonate (MJ) under
defined headspace ethylene concentrations. Kinetic studies of growth, nutrient consumption …

The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate

REB Ketchum, DM Gibson, RB Croteau… - Biotechnology and …, 1999 - Wiley Online Library
Cell suspension cultures of Taxus canadensis and Taxus cuspidata rapidly produced
paclitaxel (Taxol) and other taxoids in response to elicitation with methyl jasmonate. By …